Immunoprotective efficacy of macrophage migration inhibitory factor from Eimeria tenella and its immunoenhancement by interleukin-2
10.13200/j.cnki.cjb.004586
- VernacularTitle:柔嫩艾美耳球虫巨噬细胞迁移抑制因子的免疫保护性及白细胞介素-2对其增强作用
- Author:
Yinxue YANG
- Publication Type:Journal Article
- Keywords:
Eimeria tenella(E. tenella);
Macrophage migration inhibitory factor(MIF);
Immunoprotective efficacy;
Chicken interleukin-2
- From:
Chinese Journal of Biologicals
2026;39(06):641-648
- CountryChina
- Language:Chinese
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Abstract:
Objective To investigate the immunoprotective effects of Eimeria tenella(E.tenella) macrophage migration inhibitory factor(MIF) protein against E.tenella infection in chicks and the influence of chicken interleukin-2(IL-2)pro-tein on its immunoprotective efficacy.Methods Pichia pastorisexpression system was utilized to express E.tenella MIF protein and chicken IL-2 protein,followed by SDS-PAGE and Western blot analysis for identification.Subsequently,an immunoprotective experiment against E.tenella was conducted in chicks.A total of 135 female chicks were divided into nine groups:a non-immunized non-challenged group,a non-immunized challenged group,an empty vector group,an aluminum hydroxide adjuvant group,an IL-2 group(50 μg/chicken IL-2),an MIF group(50 μg/chicken MIF),an L-MIF + IL-2 group(25 μg/chicken MIF + 50 μg/chicken IL-2),an M-MIF + IL-2 group(50 μg/chicken MIF + 50 μg/chicken IL-2),and an H-MIF + IL-2 group(100 μg/chicken MIF + 50 μg/chicken IL-2),with 15 for each group.All groups were primed at 14 days of age by intramuscular injection of legs and boosted at 21 days of age at the same position.At 28 days of age,each bird was orally challenged with 1 × 10~5 sporulated E.tenella oocysts.The anticoccidial index(ACI) was calculated by statistical analysis of survival rate and other relevant parameters.Indirect ELISA was used to measure changes in IgY,IL-10,and IFNγlevels,and histopathological changes in cecal tissues were observed via HE staining.Results Compared with the non-immunized challenged control group,the MIF group exhibited significantly higher levels of specific IgY antibodies at 7 and14 days post-primary immunization(t = 6.215 and 7.447,respectively;each P < 0.01).No significant differences were observed in IL-10 and IFNγ levels(t = 1.970 and 0.242,respectively;each P > 0.05),with an ACI of 143.6.Compared with the MIF group,the M-MIF + IL-2 group demonstrated significantly elevated concentration of specific IgY antibodies at7 and 14 days post-primary immunization(t = 7.516 and 12.410,respectively;each P < 0.05),and significantly elevated concentrations of IL-10 and IFNγ at 21 days post-primary immunization(t = 3.338 and 2.817,respectively;each P < 0.05),accompanied by an ACI of 157.4.In the M-MIF + IL-2 group,the cell morphology was relatively complete,only a small amount of inflammatory cells infiltrated,and the intestinal gland structure was clear,which was close to the level of the non-immunized and non-challenged group.Conclusion MIF protein provides a certain degree of immunoprotection against E.tenella infection in chicks,and IL-2 protein can enhance its immunoprotective efficacy.
