Bacterial endotoxin;Recombinant C-factor method;Sabin strain inactivated poliovirus vaccine(sIPV)
10.13200/j.cnki.cjb.004512
- VernacularTitle:里氏木霉发酵过程中减少泡沫形成工艺的优化
- Author:
Zhixin GUAN
- Publication Type:Journal Article
- From:
Chinese Journal of Biologicals
2026;39(05):592-598
- CountryChina
- Language:Chinese
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Abstract:
ObjectiveTo address abnormal foam formation during liquid fermentation of Trichoderma reesei(T. reesei) for cellulase production through process optimization.MethodsUsing quadruple fermentation tank, the process was optimized through four key parameters: medium composition, seed source preparation, feeding rate and feeding methodology. Different feeding strategies were evaluated, including time-sequence feeding, dissolved oxygen(DO) process value(PV)-linked feeding, and PID algorithm-controlled user defined function(UDF) feeding. The effects of coarse wheat bran supplementation and seed culture sources(plate vs. shake flask) on the fermentation process were also examined. Cellulase activity and biomass accumulation were monitored throughout the fermentation process.ResultsBoth reduced feeding rate and coarse wheat bran supplementation effectively decreased foam formation during protein expression, preventing abnormal foaming and overflow phenomena. The PID algorithm-controlled feeding strategy enhanced microbial growth, achieving a maximum biomass of 442. 9 g/L at 145 h. Shake flask-derived seed cultures demonstrated superior enzyme production compared to plate-derived seeds, maintaining higher enzyme activity levels after 66 h of fermentation. The combination of coarse wheat bran supplementation, time-sequence feeding strategy(sequence 1), and shake flask-derived seed culture promoted optimal cellulase accumulation, reaching an enzyme activity of 60. 096 U/mL at 145 h.ConclusionAn optimized fermentation process for T.reesei was developed, which effectively controls foam formation while maintaining high enzyme productivity.The findings provide new solutions for addressing foam-related issues in T. reesei liquid fermentation and offer valuable data support for scaling up cellulase production.
