Inactivation efficacy of five disinfectants against tick-borne encephalitis virus
10.13200/j.cnki.cjb.004693
- VernacularTitle:5种消毒剂对蜱传脑炎病毒的灭活效果
- Author:
Wenjia YU
- Publication Type:Journal Article
- Keywords:
Tick-borne encephalitis virus(TBEV);
Disinfectants;
Inactivation efficacy
- From:
Chinese Journal of Biologicals
2026;39(05):540-545+553
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo evaluate the virucidal efficacy of five chemical disinfectants against tick-borne encephalitis virus(TBEV), so as to provide reference for practical application.MethodsThe virus suspension quantitative test was used to determine the inactivation effects of the five disinfectants on TBEV, including 5% Micro-Chem Plus(MCP), 75% ethanol,10% 84 disinfectant, 0. 5% compound amine, and 0. 25% compound double-chain quaternary ammonium salt. The cytotoxicity of the disinfectants on BHK-21 cells at different dilution ratios was evaluated, and the neutralizer identification test was conducted to verify the neutralization effect of the neutralizer on residual disinfectants. The virus inactivation test was performed to determine the efficacy of the disinfectants against TBEV following exposure times of 1, 2, 4 and 8 minutes.ResultsThe five disinfectants had slight cytotoxicity on BHK-21 cells at 1∶10 and 1∶100 dilution ratios, but no obvious cytotoxicity at 1∶1 000 and higher dilution ratios. The used neutralizer could effectively terminate the effect of each disinfectant, and the neutralizer and its neutralization products did not affect the virus titer. All the five disinfectants achieved an average inactivation log value ≥ 4. 00 against TBEV after contact times of 1, 2, 4 and 8 minutes, which could completely inactivate the virus.Conclusion5% MCP, 75% ethanol, 10% 84 disinfectant, 0. 5% compound amine, and 0. 25% compound double-chain quaternary ammonium salt all have killing effects on TBEV, and are safe at 1∶1 000 and higher dilution ratios.The used neutralizer can effectively ensure the accuracy of experimental operations after disinfection.
