Application of HTS2 Technology in Traditional Chinese Medicine and Formulas： A Review
10.13422/j.cnki.syfjx.20260112
- VernacularTitle:HTS2技术在中药与方剂研究中的应用进展
- Author:
Xiaohong YI
1
;
Yumei WANG
1
;
Yuhui CHEN
1
;
Dong WANG
1
;
Lijun HUANG
1
Author Information
1. School of Basic Medical Sciences，Chengdu University of Traditional Chinese Medicine， Chengdu 610030，China
- Publication Type:Journal Article
- Keywords:
high-throughput sequencing-based high-throughput screening （HTS2） technology;
characteristic gene expression signature;
traditional Chinese medicine;
drug discovery
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2026;32(14):40-49
- CountryChina
- Language:Chinese
-
Abstract:
High-throughput sequencing-based high-throughput screening （HTS2） technology， as a new advancement in the field of high-throughput biotechnology， is the world's first technology to integrate high-throughput sequencing into large-scale drug screening and target discovery. The artificially designed DNA probes were bound to the undetermined mRNAs of thousands of genes in cell lysates， and then the probes were ligated with ligases. The large-scale simultaneous detection of gene expression changes in thousands of drug-treated cell samples was performed using barcoding， automated operating platforms， and high-throughput sequencers. This technology enables high-throughput identification of drugs that significantly perturb the gene expression profiles characteristic of diseases. It can also take gene expression signature as the readout and exert great high-throughput advantages in the screening of multi-drug， multi-component， and multi-target drugs， as well as the research on complex mechanisms. Therefore， it is particularly suitable for elucidating the multi-target mechanisms of traditional Chinese medicine and identifying its multi-effective components. Its main technical advantages include high throughput， automation， and low cost. In recent years， HTS2 technology has yielded important achievements in the elucidation of the mechanism of action of traditional Chinese medicine formulas， the scientific connotation analysis of the regional characteristics of traditional Chinese medicine， the targeted isolation of active compounds of traditional Chinese medicine， and the discovery of novel pharmacological functions of monomeric compounds of traditional Chinese medicine. In the era of artificial intelligence， HTS2 technology will serve as a powerful tool for generating high-quality， original big data of traditional Chinese medicine， providing core data support and promoting AI-driven traditional Chinese medicine research. Ultimately， HTS2 technology offers new strategies and critical data support for deeply analyzing the scientific connotation of traditional Chinese medicine and discovering novel traditional Chinese medicine-based drugs， thereby accelerating the modernization and internationalization of traditional Chinese medicine in China.
