How to select appropriate human-derived antiserum for absorption and elution test
10.13303/j.cjbt.issn.1004-549x.2026.05.013
- VernacularTitle:吸收放散试验选择人源抗血清的探讨
- Author:
Dandan CHEN
1
;
Xiaoyan LI
1
;
Xuan ZENG
1
Author Information
1. Department of Blood Transfusion, Jiangxi Maternal and Child Health Hospital, Maternal and Child Health Hospital of Nanchang Medical College, Nanchang 330088, China
- Publication Type:Journal Article
- Keywords:
ABO blood group;
absorption and elution test;
weak antigen;
human antiserum;
gene sequencing
- From:
Chinese Journal of Blood Transfusion
2026;39(5):667-672
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the importance of selecting appropriate human antiserum for absorption and elution tests by analyzing two cases in which weak A antigens were detected using this method. Methods: The microcolumn agglutination and tube methods were used to perform ABO blood group typing of the patients. Absorption and elution tests were conducted to detect weak A antigens on red blood cells. Molecular biological methods were employed for genotyping. Results: Serological tests showed that the forward typing results of the two patients were O and B, respectively, and weak anti-A1 antibodies were present in their sera. When O-type human high-titer (anti-A≥256) serum was used for absorption and elution tests, patient 1 eluted anti-A, confirming the presence of weak A antigens on their red blood cells; patient 2 eluted anti-A and anti-AB simultaneously. When B-type human antiserum was used for absorption and elution, patient 2 eluted anti-A, confirming the presence of weak A antigens on their red blood cells as well. Gene sequencing results showed that the genotypes of the two patients were ABO
Aw. 04/ABO
O. 01. 02 and ABO
AEL (c. 410C>T)/ABO
B.01, respectively. Conclusion: When absorption and elution tests are needed for weak A/B antigens alone on red blood cells, O-type human high-titer serum can be prioritized. However, when both A and B antigens are present, the human antiserum selected for absorption and elution tests should only react with the weak antigen and not with the normally expressed antigen.
