Shaoyaotang Regulates TLR4/MyD88/NF-κB Signaling Pathway to Protect Intestinal Mucosal Barrier in Ulcerative Colitis
10.13422/j.cnki.syfjx.20260506
- VernacularTitle:芍药汤调节TLR4/MyD88/NF-κB信号通路保护溃疡性结肠炎肠黏膜屏障的作用机制
- Author:
Dongsheng WU
1
;
Yu ZHANG
1
;
Wenjing QUAN
2
;
Wanqing XIONG
2
;
Bo ZOU
1
;
Youwei XIAO
1
;
Ruoru HUANG
1
;
Yan GONG
1
;
Hui CAO
1
Author Information
1. The First Hospital of Hunan University of Chinese Medicine， Changsha 410007， China
2. Anhui University of Chinese Medicine， Hefei 230012， China
- Publication Type:Journal Article
- Keywords:
ulcerative colitis;
Shaoyaotang;
Toll-like receptor 4 （TLR4）/myelodifferentiation factor 88 （MyD88）/nuclear factor κB （NF-κB）;
intestinal mucosal barrier
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2026;32(13):69-75
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the role of the Toll-like receptor 4 （TLR4）/myeloid differentiation factor 88 （MyD88）/nuclear factor-κB （NF-κB） signaling pathway in intestinal mucosal barrier damage in ulcerative colitis， as well as the intervention mechanism of Shaoyaotang. MethodsSixty SD rats were allocated into a blank group， a model group， a mesalazine （0.42 g·kg-1） group， and low-， medium-， and high-dose （11.1， 22.2， 44.4 g·kg-1， respectively） Shaoyaotang groups. A model of ulcerative colitis was induced by 2，4，6-trinitrobenzenesulfonic acid （TNBS）. After successful modeling， rats were administrated with corresponding agents via gavage for 7 days. Changes in colon length and colon weight were observed. Hematoxylin-eosin staining was performed to examine the pathological changes of the colon， and immunohistochemistry was employed to detect the expression of the inflammatory cytokine interleukin-8 （IL-8）， cyclooxygenase-2 （COX-2）， junction adhesion molecule-1 （JAM-1）， and claudin-1 in the colon. Western blot analysis was performed to determine the protein levels of TLR4， MyD88， and NF-κB in the colon. ResultsCompared with the blank group， the model group showed elevated DAI score （P<0.01）， reduced colon length and colon weight （P<0.01）， down-regulated protein levels of JAM-1 and claudin-1 （P<0.01）， and up-regulated protein levels of IL-8， COX-2， TLR4， MyD88， and NF-κB p65 （P<0.01） in the colon tissue. Compared with the model group， each treatment group showed decreased DAI score （P<0.05， P<0.01）， increased colon length and colon weight （P<0.05， P<0.01）， up-regulated protein levels of JAM-1 and claudin-1 （P<0.01）， and down-regulated protein levels of IL-8， COX-2， TLR4， MyD88， and NF-κB p65 （P<0.01） in the colon tissue. ConclusionShaoyaotang alleviates intestinal inflammation and intestinal mucosal damage to protect intestinal barrier integrity by regulating the TLR4/MyD88/NF-κB signaling pathway.
