Shaoyaotang Ameliorates Ulcerative Colitis by Regulating miR-155-5p
10.13422/j.cnki.syfjx.20260401
- VernacularTitle:芍药汤调控miR-155-5p改善溃疡性结肠炎的机制
- Author:
Ruoru HUANG
1
;
Bo ZOU
1
;
Yu ZHANG
1
;
Yiqian YU
1
;
Qi CHENG
1
;
Youwei XIAO
1
;
Jiachun XIONG
1
;
Yan GONG
1
;
Dongshen WU
1
;
Hui CAO
1
Author Information
1. The First Hospital of Hunan University of Chinese Medicine， Changsha 410007， China
- Publication Type:Journal Article
- Keywords:
Shaoyaotang;
ulcerative colitis;
microRNA-155-5p;
Janus kinase1/signal transducer and activator of transcription 1（JAK1/STAT1） signaling pathway;
intestinal inflammation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2026;32(13):61-68
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the role of microRNA-155-5p （miR-155-5p） in ulcerative colitis （UC） and study the molecular mechanism of Shaoyaotang in the treatment of UC by regulating miR-155-5p. MethodsForty-eight SPF-grade male C57BL/6 mice were selected and assigned via the random number table method into 6 groups （n=8）： A blank control group， a model group， a mesalazine （0.39 g·kg-1） group， a Shaoyaotang （31.08 g·kg-1） group， a Janus kinase 1 （JAK1） inhibitor （baricitinib， 10 mg·kg-1） group， and a Shaoyaotang combined with inhibitor （baricitinib 10 mg·kg-1 + Shaoyaotang 31.08 g·kg-1） group. After successful modeling of UC by gavage of 3% dextran sulphate sodium solution， each group received corresponding drug intervention for 7 days. Shaoyaotang and mesalazine were administered by gavage， and baricitinib by intraperitoneal injection. Twenty-four hours after the last administration， mice were anesthetized by intraperitoneal injection of pentobarbital sodium， and blood was collected for determination of white blood cell count and erythrocyte sedimentation rate （ESR）. Mice were then sacrificed for measurement of colon length. Hematoxylin-eosin staining was used to observe colonic pathological changes and perform pathological scoring. Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） was employed to determine the relative expression of miR-155-5p in the colonic tissue， and Western blot was used to determine the protein levels of JAK1， phosphorylated JAK1 （p-JAK1）， suppressor of cytokine signaling 1 （SOCS1）， signal transducer and activator of transcription 1 （STAT1）， and phosphorylated STAT1 （p-STAT1）. ResultsCompared with the blank control group， the model group showed increased disease activity index （DAI） score and pathological score， shortened colon， upregulated relative expression of miR-155-5p and protein levels of p-JAK1 and p-STAT1， downregulated protein level of SOCS1 in the colonic tissue， prolonged time of erythrocyte sedimentation， and increased white blood cell count （P<0.01）. Compared with the model group， all drug-treated groups exhibited improvements in the above indicators （P<0.01）. Moreover， the Shaoyaotang group showed better therapeutic effects than the mesalazine group in regulating miR-155-5p expression， related protein levels， DAI score， and colonic pathological score （P<0.01）. ConclusionShaoyaotang may downregulate miR-155-5p to relieve its inhibition on SOCS1， thereby suppressing the excessive activation of the JAK1/STAT1 signaling pathway and ultimately alleviating intestinal inflammatory damage.
