Reactive oxygen species mediate promoting effect of fullerene C60 on DHPN-induced lung tumors
10.19405/j.cnki.issn1000–1492.2026.04.011
- VernacularTitle:氧自由基介导富勒烯C60对DHPN诱导肺肿瘤的促进作用
- Author:
Wen ZHANG
1
;
Jiegou XU
1
Author Information
1. Dept of Immunology， Anhui Medical University， Hefei 230032
- Publication Type:Journal Article
- Keywords:
fullerene C60;
lung tumor;
N-bis （2-hydroxypropyl） nitrosamine;
intratracheal spraying;
Fisher 344 rats
- From:
Acta Universitatis Medicinalis Anhui
2026;61(4):677-681
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate whether fullerene C60 administered by intratracheal spraying promotes N-bis （2-hydroxypropyl） nitrosamine （DHPN） induced-lung tumor development and the underlying mechanisms. MethodsA rat lung tumor model was induced by the DHPN drinking water method. Eight-week-old male Fisher 344 rats were randomly divided into 5 groups （n = 15）： DHPN+Veh group （0.2% DHPN + vehicle solution）， DHPN+C60-L group （0.2% DHPN + 250 μg/mL C60）， DHPN+C60-H group （0.2% DHPN + 500 μg/mL C60）， DHPN group （0.2% DHPN）， and C60-H group （normal drinking water + 500 μg/mL C60）. 0.2% DHPN in drinking water was given to initiate carcinogenesis， and 2 weeks later， different concentrations of fullerene C60 were administered by intratracheal spraying every two weeks for a total of 20 times. Two weeks after the last spraying， the incidence， number and size of lung tumors were statistically analyzed. In another mechanism animal experiment， 8-week-old male Fisher rats were divided into two groups with 5 rats in each group： C60 group and the control vehicle group. 500 μg/mL C60 or the control vehicle intratracheally sprayed every two days for a total of 5 times. Six hours after the last spraying， the lungs were removed and used for observation of C60 distribution and for detection of SOD， 8-OHdG and cytokines in lung tissues. ResultsNo lung tumorigenesis was observed in the C60-H group； the incidence， number and size of lung tumors in the DHPN+C60-H group were significantly higher than those in the DHPN+Veh group （P<0.05， P<0.001， P<0.05） and the DHPN group （P<0.01， P<0.001， P<0.01）， respectively. In additional animal experiments designed for mechanistic investigation， the levels of SOD and 8-OHdG in the experimental group were markedly elevated compared with the control group （both P<0.001）， with statistically significant differences. ConclusionIntratracheal nebulization of high-concentration fullerene C60 promoted DHPN-induced lung tumorigenesis； C60 led to an increase in reactive oxygen species （ROS） production， thereby elevating the potential for gene mutation. This may represent one of the mechanisms underlying C60-induced promotion of lung tumorigenesis.
