Effects of different concentrations and treatment durations of hyaluronidase on mouse zygote development and blastocyst formation
10.19405/j.cnki.issn1000–1492.2026.03.021
- VernacularTitle:不同浓度和处理时长的透明质酸酶对小鼠受精卵发育和囊胚形成的影响
- Author:
Jie ZHANG
1
;
Hua WANG
2
;
Di YANG
1
;
Jinfang LU
1
;
Jicong ZHANG
1
Author Information
1. School of Biomedical Engineering， Anhui Medical University， Hefei 230012
2. Hefei Innovation Research Institute of Beihang University， Hefei 230012
- Publication Type:Journal Article
- Keywords:
hyaluronidase;
concentration;
treatment duration;
fertilized egg;
blastocyst development;
mouse
- From:
Acta Universitatis Medicinalis Anhui
2026;61(3):540-545
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo define hyaluronidase treatment parameters that efficiently remove the extracellular matrix from zygotes without impairing embryo development， thereby providing a basis for standardized assisted reproductive procedures. MethodsMouse zygotes were treated with hyaluronidase at 40， 80， or 120 IU/mL for 60， 90， or 120 s. Following treatment， embryos were cultured in Krebs-ringer bicarbonate-based solution for organismal media （KSOM） medium， and blastocyst formation was monitored using a Time-lapse imaging system. ResultsA concentration of 40 IU/mL was insufficient for effective matrix removal， whereas 120 IU/mL markedly reduced blastocyst formation. In contrast， 80 IU/mL achieved efficient matrix clearance with minimal impact on development. Blastocyst rates were comparable between the 60 s and 90 s groups， with a slight advantage for the 60 s under the experimental conditions. ConclusionHyaluronidase treatment at 80 IU/mL for 60 s represents optimal processing conditions for mouse zygotes. These findings provide experimental basis and standardized reference for embryo handling in assisted reproductive technologies.
