The expression and mechanistic study of Y‑box binding protein‑1 in oral squamous cell carcinoma
10.19405/j.cnki.issn1000–1492.2026.03.019
- VernacularTitle:Y-box结合蛋白-1 在口腔鳞癌中的表达及作用机制
- Author:
Yi ZHANG
1
;
Xiao YU
1
;
Wei SHAO
2
;
Xin CHEN
1
Author Information
1. College Hospital of Stomatology， Anhui Medical University， Key Lab. of Oral Diseases， Research of Anhui Province， Hefei 230032
2. Department of Pathogen Biology， School of Basic Medicine， Anhui Medical University， Hefei 230032
- Publication Type:Journal Article
- Keywords:
Y-box binding protein-1;
oral squamous cell carcinoma;
proliferation;
migration;
invasion;
PI3K/AKT signaling pathway
- From:
Acta Universitatis Medicinalis Anhui
2026;61(3):524-532
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo analyze the expression level and clinical significance of Y‑box binding protein‑1（YB1） in tissues and cells of oral squamous cell carcinoma （OSCC）. MethodsBioinformatics analysis， immunohistochemistry， Western blot and qRT-PCR were used to detect the expression of YB1 in OSCC tissues， and the prognosis and survival analysis were performed. The effects of YB1 on the proliferation， migration and invasion of OSCC cells were evaluated by CCK-8， scratch assay and Transwell assay， and the expression changes of phosphoinositide 3-kinase/protein kinase B （PI3K/AKT） signaling pathway and epithelial-mesenchymal transition （EMT） related markers after YB1 knockdown were detected by Western blot. ResultsBioinformation analysis showed that the expression level of YB1 in OSCC samples was higher than that in normal samples （P0.001）， and its expression was correlated with gender （P=0.022） and tumor differentiation degree （P0.001）. Kaplan-Meier plotter survival analysis showed that the survival rate of YB1 was low （Log-rank P=0.012）. Immunohistochemistry， Western blot and qRT-PCR experiments verified that the expression level of YB1 in OSCC tissues was higher than that of normal tissues. The results of cell experiments showed that knockdown of YB1 inhibited the malignant biological behaviors such as proliferation， migration and invasion of OSCC cells. Western blot results showed that the knockdown of YB1 could reduce the protein expression levels of p-PI3K and p-AKT， and inhibit EMT. ConclusionYB1 is highly expressed in tissues and cells in OSCC， and reveals the important role of the YB1/PI3K/AKT axis in the process of EMT of OSCC， which is expected to become a new tumor marker for early diagnosis and treatment and prognosis evaluation of OSCC.
