The study of m6A methylation-related proteins in the prefrontal cortex of PTSD mice
10.19405/j.cnki.issn1000–1492.2026.03.015
- VernacularTitle:PTSD小鼠前额叶区m6A甲基化相关蛋白的研究
- Author:
Jiaying LU
1
;
Luodong YANG
1
;
Keke LU
1
;
Wenlong XIN
1
;
Bin LI
1
;
Qulong LI
1
;
Guiqing ZHANG
1
Author Information
1. Department of Clinical Psychology， First Affiliated Hospital of Shihezi University， Shihezi 832000
- Publication Type:Journal Article
- Keywords:
post-traumatic stress disorder;
prefrontal cortex;
SPSS model;
m6A-related proteins;
neurons
- From:
Acta Universitatis Medicinalis Anhui
2026;61(3):495-500
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the expression of prefrontal cortical neurons， methyltransferase-like 3 （METTL3）， fat mass and obesity-associated gene （FTO）， and AlkB homolog 5 （ALKBH5） proteins in a mouse model of post-traumatic stress disorder （PTSD）. MethodsA PTSD mouse model was established using a single prolonged stress and foot shock stimulation （SPSS） method. The despair， anxiety， and learning and memory functions of PTSD mice were assessed through the open field test， Y-maze test， and forced swimming test. Neuronal damage was detected via HE and Nissl staining. The expression levels of METTL3， FTO， ALKBH5， and neuronal nuclear protein （NEUN） were assessed by Western blot and immunofluorescence staining. ResultsCompared to control group， PTSD mice subjected to SPSS exhibited signs of despair， anxiety， and impaired learning and memory. HE and Nissl staining results showed neuronal damage in the prefrontal cortex of PTSD mice. Western blot and immunofluorescence staining results showed that the expression of the m6A-related proteins METTL3 and FTO decreased， while the expression of ALKBH5 increased in the prefrontal cortex. Additionally， NEUN protein levels showed a declining trend. ConclusionThe pathogenesis of PTSD may be associated with neuronal damage in the prefrontal cortex and alterations in m6A methylation proteins.
