Expression and clinical significance of ARTN in prostate cancer
10.19405/j.cnki.issn1000–1492.2026.03.009
- VernacularTitle:ARTN在前列腺癌中的表达及临床意义
- Author:
Rong LI
1
;
Junfeng JING
1
;
Can WEI
1
Author Information
1. ［Department of Urology， Hefei Hospital Affiliated to Anhui Medical University （The Second People's Hospital of Hefei）， Hefei 230011］
- Publication Type:Journal Article
- Keywords:
artemin;
prostate cancer;
survival rate;
prognosis;
epithelial-mesenchymal transition;
biomarker
- From:
Acta Universitatis Medicinalis Anhui
2026;61(3):448-454
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the clinical significance of artemin （ARTN） expression in prostate cancer （PCa） tissues and its impact on the malignant behavior of PCa cell lines. MethodsImmunohistochemistry was used to detect the expression of ARTN protein in 40 benign prostate tissues and 91 PCa tissues， and its relationship with the clinical and pathological characteristics of PCa was analyzed. PCa stable cell lines with ARTN knockdown were constructed， and the effects of ARTN on the proliferation， migration， and invasion ability of PCa cells were detected via CCK-8 cell proliferation assay and Transwell assay. Western blot assay was used to detect the effect of ARTN on the expression of epithelial-mesenchymal transition （EMT） related markers E-cadherin， N-cadherin， Vimentin， and Snail family transcription inhibitory factor 1 （Snail-1）. ResultsARTN was highly expressed in PCa and correlated with Gleason score， local lymph node metastasis， and local nerve invasion （P0.05）. Survival analysis showed a statistically significant difference in survival rates between ARTN positive and negative patients （P=0.027）. The results of CCK-8 and Transwell assay showed that the knockdown of ARTN could inhibit the proliferation， migration， and invasion ability of PCa cells （all P0.05）. Western blot results showed that the knockdown of ARTN upregulated the epithelial marker E-cadherin in PCa cells， while the mesenchymal markers N-cadherin， Snail-1， and Vimentin were downregulated. ConclusionARTN is highly expressed in PCa and can promote the proliferation， migration， invasion ability of PCa cells， as well as increasing EMT levels in PCa cells， suggesting it may be a potential target for the diagnosis and treatment of PCa.
