The effect of Chuju total flavonoids on the current and protein expression of BKCa channels in rat middle cerebral artery VSMC
10.19405/j.cnki.issn1000–1492.2026.03.001
- VernacularTitle:滁菊总黄酮对大鼠大脑中动脉VSMC的BKCa通道的电流及蛋白表达的影响
- Author:
Yuwen LI
1
;
Xiao WANG
1
;
Miao WU
1
;
Shuo CHEN
2
;
Zhiwu CHEN
1
Author Information
1. College of Pharmaceutical Sciences， Anhui Medical University， Hefei 230032
2. School of Traditional Chinese Medicine， Anhui University of Traditional Chinese Medicine， Hefei 230012
- Publication Type:Journal Article
- Keywords:
Chuju total flavonoids;
large-conductance Ca22+ -activated K+;
vascular smooth muscle cells;
hydrogen sulfide;
whole cell patch clamp;
Western blot
- From:
Acta Universitatis Medicinalis Anhui
2026;61(3):387-394
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the relationship between the vasodilation and hydrogen sulfide （H2S） mechanism of total flavonoids of chrysanthemum （TFCC） and the large conductance Ca2+- activated K+ （BKCa） channels on vascular smooth muscle cells （VSMCs） of the middle cerebral artery in rats. In addition， this study will also investigate the effect of TFCC on the expression of BKCa channel alpha protein in rat middle cerebral artery VSMCs. MethodsThe primary method employed was acute digestion to isolate VSMCs from the middle cerebral artery of rats； whole-cell patch-clamp techniques were used to measure BKCa channel currents； primary tissue adherence culture was utilized to cultivate VSMCs from the middle cerebral artery of rats； Western blot were employed to determine protein expression levels. ResultsIn whole-cell patch-clamp experiments， both the H2S donor NaHS （100 μmol/L） and endogenous H2S enhanced BKCa channel currents. TFCC （30， 90， and 270 mg/L） also activatedBKCa channels and exhibited a certain concentration-dependent effect. Even after blocking endogenous H2S production， TFCC （270 mg/L） still activated BKCa channels in VSMCs of the middle cerebral artery in rats. In Western blot experiments， the α-subunit of BKCa channel proteins was expressed in all groups of cells， but TFCC （30， 90， and 270 mg/L） and inhibitor IBTX group did not affect the expression of channel protein content.Conclusion TFCC can promote the opening of BKCa channels by promoting the generation of endogenous H2S， or directly activate BKCa channels， thereby playing a role in relaxing cerebral blood vessels. However， TFCC had no significant effect on the expression of BKCa channel proteins.
