Determination of lamotrigine in human plasma by central cutting two-dimensional liquid chromatography
10.12206/j.issn.2097-2024.202306020
- VernacularTitle:中心切割二维液相色谱法测定人血浆中拉莫三嗪血药浓度的研究
- Author:
Junrong LIN
1
;
Minxin ZHANG
1
;
Xuemei ZHAO
1
;
Aiwen HUANG
1
Author Information
1. Department of Clinical Pharmacy, No.900 Hospital of Joint Logistic Support Force, Fuzhou 350025, China.
- Publication Type:Researchreports
- Keywords:
lamotrigine;
plasma concentration;
two-dimensional liquid chromatography;
central cutting
- From:
Journal of Pharmaceutical Practice and Service
2026;44(1):53-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a central cutting two-dimensional liquid chromatography for the determination of lamotrigine in human plasma. Methods External standard method was used. The first dimensional chromatographic column: SNCB（T）-1A（silica gel, 4.6 mm×50 mm, 5 μm）, mobile phase A:VCV-1D mobile phase, flow rate: 0.4 ml/min; mobile phase B: water, flow rate: 1.0 ml/min; second dimensional chromatographic column: Symmetry C18 （4.6 mm×250 mm, 5 μm）, mobile phase: acetonitrile-10 mmol/L ammonium acetate solution（V/V=25∶75）, flow rate: 1.0 ml/min；Intermediate column: SBX 4-MA（resin, 3.0 mm×10 mm, 5µm）. The UV detection wavelength：306 nm, the column temperature：45 ℃, and the injection volume：200 μl. Results The linear range of lamotrigine was 1.24-39.50 μg/ml, the lower limit of quantification was 1.24 μg/ml, the detection limit was 0.02 μg/ml, the intra-day precision RSD was less than 5%, the day-to-day precision RSD was less than 10%, the variation of intra-day accuracy ranged from 102.17% to 111.17%, and the daytime accuracy variation ranged from 99.80% to 107.31% the recovery RSD was less than 5%, and the variation range was 89.95% -96.16%. After 24 hours storage at room temperature, repeated freezing and thawing for 3 times and storage at −40 ℃ for 2 weeks, the ratio of the measured value/labeled value ranged from 87.01% to 115.88%. Conclusion In this study, a method with simple operation, good stability, high sensitivity and good reproducibility was established, which could be suitable for clinical monitoring of blood concentration of lamotrigine and provide reliable monitoring data support for clinical individualized medication guidance.
