Study on quality standard of Andrographis paniculata standard decoction based on UPLC characteristic chromatogram and quantitative analysis of multi-components by single marker
10.12206/j.issn.2097-2024.202310021
- VernacularTitle:基于UPLC特征图谱和一测多评法的穿心莲标准汤剂质量标准研究
- Author:
Zhiling MA
1
;
Xiaoya WANG
2
;
Weibo DU
2
;
Zhiqiang ZHANG
2
;
Jianmei SHEN
2
;
Yan LIU
3
Author Information
1. Taiyuan Xiaodian District People’s Hospital, Taiyuan 030032, China;Beijing Kangrentang Pharmaceutical Co., Ltd, Beijing 101301, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
2. Beijing Kangrentang Pharmaceutical Co., Ltd, Beijing 101301, China.
3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Researchreports
- Keywords:
Andrographis paniculata;
standard decoction;
UPLC;
QAMS;
characteristic chromatogram;
quality standard
- From:
Journal of Pharmaceutical Practice and Service
2026;44(1):46-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish quality evaluation method of Andrographis paniculata standard decoction by UPLC. Methods 21 batches of Andrographis paniculata standard decoctions were prepared according to the standardization method of TCM decoction pieces. The UPLC characteristic chromatograms analysis method was established. With andrographolide as a reference, quantitative analysis of multi-components by single marker （QAMS） was established for new neoandrographolide, 14 deoxyandrographolide and dehydrated andrographolide. The results were compared with the external standard method （ESM） to determine the accuracy of the method. Results Similarity Evaluation System for Chromatographic Fingerprint of TCM （2012 edition） was used to analyze and compare the characteristic chromatograms, and seven common peaks were determined and five were identified including luteolin-7-O-β-D-glucuronide, andrographolide, neoandrographolide, 14-deoxyandrographolide and dehydroandrographolide. The RSDs of content results of each component by QAMS and ESM were all within 3%. Conclusion The determination method was reliable and accurate, which could be used to reflect the intrinsic quality of Andrographis paniculata standard decoction comprehensively and provide the basis for the quality evaluation of Andrographis paniculata formula granules and other preparations.
