The mechanism of nicotinamide combined with amphotericin B against Candida albicans based on metabolomics technology
10.12206/j.issn.2097-2024.202307034
- VernacularTitle:基于代谢组学技术的烟酰胺协同两性霉素B抑制白念珠菌的作用机制研究
- Author:
Lizhi WAN
1
;
Jinghan WANG
1
;
Chunrong WU
1
;
Ling LI
1
Author Information
1. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
- Publication Type:Originalarticles
- Keywords:
gas chromatography-mass spectrometry;
amphotericin B;
nicotinamide;
candida albicans;
metabolomics
- From:
Journal of Pharmaceutical Practice and Service
2026;44(1):20-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential mechanism of nicotinamide combined with amphotericin B against Candida albicans based on metabolomics. Methods The intracellular metabolites of C. albicans intervened by different drugs including NAM, AmB, and their combination with a proper concentration were analyzed by gas chromatography-mass spectrometry. The differential metabolites were screened by multivariate statistical analysis and identified by searching the NIST database. Results Compared with the control group, the NAM intervention group was hardly separated from it, while the AmB group and NAM+AmB group showed a clear trend of separation. Under the intervention of AmB, 23 metabolites significantly changed compared with the control group, and 28 metabolites remarkably changed after NAM+AmB intervention, including amino acids, organic acids, sugars and other components. Conclusion NAM, as an endogenous metabolite of C. albicans, combined with AmB could enhance the effects of AmB in the original metabolic pathway and changed it to a certain extent. It was speculated that AmB combined with NAM may pose more antifungal effect on Candida albicans by regulating the tricarboxylic acid cycle，interfering with amino acid metabolism and influencing polyamine synthesis.
