Influence of high copper load on neurobehavior and the sirtuin 3/peroxisome proliferator-activated receptor-γ coactivator-1α signaling pathway in rats
10.19845/j.cnki.zfysjjbzz.2026.0044
- VernacularTitle:高铜负荷对大鼠神经行为和SIRT3/PGC-1α信号通路的影响
- Author:
Shan JIANG
1
;
Ting GAO
1
Author Information
1. The First Clinical Medical College of Anhui University of Chinese Medicine， Hefei 230038， China
- Publication Type:Journal Article
- Keywords:
Hepatolenticular degeneration;
Neurobehavior in rats;
Sirtuin 3/peroxisome proliferator-activated receptor-γ coactivator-1α;
Signaling pathway
- From:
Journal of Apoplexy and Nervous Diseases
2026;43(3):254-258
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of high copper load on neurobehavior in rats and its possible mechanism of action based on the deacetylase sirtuin 3 （SIRT3）/peroxisome proliferator-activated receptor-γ coactivator-1α （PGC-1α） signaling pathway. Methods A total of 50 adult Sprague-Dawley rats were randomly divided into model group and blank group， with 25 rats in each group. The rats in the model group were fed with 1 g/kg high-copper diet and 0.185% copper sulfate solution （prepared using deionized water） to establish a rat model of high copper load， and after 12 weeks， the shuttle box test and the pole-climbing test were used to observe neurobehavioral changes， while Western blotting and immunofluorescent staining were used to measure the protein expression levels of SIRT3 and PGC-1α in hippocampal tissue. Results The shuttle box test showed that compared with the blank group， the model group had a significant reduction in the number of times of active avoidance reaction after stimulation （P<0.01） and significant increases in the times of active avoidance reaction and passive avoidance reaction （P<0.01）；the pole-climbing test showed that compared with the blank group， the model group had a significant increase in pole-climbing time（P<0.01）. Western blotting and immunofluorescent staining showed that compared with the blank group， the model group had significant reductions in the protein expression levels of SIRT3 and PGC-1α in hippocampal tissue （P<0.05）. Conclusion High copper load has a marked influence on the neurobehavior of rats， possibly by regulating the expression of the SIRT3/PGC-1α signal pathway in hippocampal neurons.
- Full text:2026061108300034871高铜负荷对大鼠神经行为和SIRT3_PGC-1α信号通路的影响.pdf
