Effects of Danggui Wuji granules on 16S r DNA, metagenome, and metabolome in BDS mice

Hanhui HUANG; Yurong DENG; Shisheng CHEN; Zongyuan LI; Xiaomin LI; Jianhui SUN; Liyu HAO; Zeyue YU; Aoao WANG; Jianliang LI; Hairu HUO; Guimin LIU; Hongmei LI

Return

Effects of Danggui Wuji granules on 16S r DNA, metagenome, and metabolome in BDS mice

Author: Hanhui HUANG ¹ ; Yurong DENG ¹ ; Shisheng CHEN ² ; Zongyuan LI ³ ; Xiaomin LI ² ; Jianhui SUN ¹ ; Liyu HAO ¹ ; Zeyue YU ¹ ; Aoao WANG ¹ ; Jianliang LI ¹ ; Hairu HUO ¹ ; Guimin LIU ¹ ; Hongmei LI ¹
Author Information

1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China
2. PERFECT Daily Necessities Co, Ltd, Zhongshan Guangdong, China
3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China; Tianjin University of Traditional Chinese Medicine, Tianjin, China
Publication Type:Journal Article
Keywords: Blood deficiency syndrome; Danggui Wuji granules; 16S r DNA sequencing; Metagenome; Metabolomics; Angelicae Sinensis Radix
From: Science of Traditional Chinese Medicine 2024;2(1):29-36
CountryChina
Language:English
Abstract: Blood deficiency syndrome (BDS) refers to a pathological state with blood dysfunction and organ dystrophy in traditional Chinese medicine. Danggui Wuji granules (DWG) was developed from a formula containing Angelicae Sinensis Radix and Musculus et Os Galli Domestici. Herein, we investigated the mechanism of DWG in treating BDS by modulating gut microbiota. We found that DWG protected mice from BDS by elevating the levels of red blood cell count, hemoglobin, and hematocrit in peripheral blood and increasing the erythrocyte membrane Na⁺-K⁺-ATPase activity. Danggui Wuji granules changed the composition and metabolites of colonic flora. Notably, Lactobacillus, Muribaculaceae, and Alistipes were the main genera showing changes after DWG treatment. Our findings revealed that DWG presented a positive therapeutic effect on BDS in mice by regulating the gut microbiota and metabolites. The protective mechanism of DWG was associated with pathways such as metabolic pathways, biosynthesis of secondary metabolites, ABC transporters, ribosome, thyroid hormone synthesis, lysine degradation, galactose metabolism, tyrosine metabolism, etc.