Ultrasonic-assisted alkaline dilution-inductively coupled plasma mass spectrometry for the determination of total bromine in whole blood
10.3760/cma.j.cn121094-20240111-00010
- VernacularTitle:全血中总溴测定的超声辅助碱稀释-电感耦合等离子体质谱法
- Author:
Yan GONG
1
;
Yukang WU
1
;
Yuanhua MENG
1
;
Xiaofeng CHEN
1
Author Information
1. 无锡市疾病预防控制中心（南京医科大学附属无锡疾病预防控制中心）理化检验科，无锡 214023
- Publication Type:Journal Article
- Keywords:
Mass spectometry;
Whole blood;
Bromine;
Inductively coupled plasma mass spectrometry;
Ultrasonic-assisted
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2025;43(1):68-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an inductively coupled plasma mass spectrometry (ICP-MS) method for the rapid determination of total bromine in whole blood, and to provide technical support for the monitoring of bromine exposure in occupational populations.Methods:In September 2023, 0.25 ml of whole blood sample was added with 0.25% tetramethylammonium hydroxide solution to a volume of 5.0 ml, ultrasonicated and homogenized, and then quantified by ICP-MS with rhodium solution as the online internal standard solution.Results:The linear relationship of bromine in blood was good within the range of 0~10.00 mg/L, with a correlation coefficient ( r) >0.999. The limit of detection of the method was 0.07 mg/L, and the quantification limit was 0.22 mg/L. The recoveries of the total bromine in whole blood were in the range of 95.5%-102.9%, and the relative standard deviations (RSDs) were in the range of 3.1%-4.7% ( n=7) . Conclusion:An ICP-MS method was established for the rapid determination of total bromine in whole blood, which is accurate, simple, rapid, highly automated, and can be used for the determination of total bromine in whole blood of occupationally exposed people.
