Construction of a realistic tumor cell surface model and dose estimation under irradiation conditions
10.3760/cma.j.cn113030-20240810-00310
- VernacularTitle:真实肿瘤细胞曲面模型的构建及其辐照条件的剂量分布研究
- Author:
Yunan GAO
1
;
Yuan ZHUANG
1
;
Jiayu WU
1
;
Jiayue LIU
1
;
Yixing CHEN
1
Author Information
1. 复旦大学附属中山医院放疗科，上海 200032
- Publication Type:Journal Article
- Keywords:
Glioma;
Cells;
Dosimetry;
Monte Carlo simulation;
Cell surface model
- From:
Chinese Journal of Radiation Oncology
2025;34(10):1014-1019
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct a realistic surface model of human glioma T98G cells, aiming to enhance the accuracy of dose assessment at the cellular level in radiotherapy.Methods:Three-dimensional tomographic images of T98G cells were acquired using a laser confocal microscope. Subsequently, after cropping via MATLAB software and conversion to the DICOM format, the Amira and Meshmixer softwares were employed to repair and reconstruct the authentic curved - surface models of the cell nucleus and cytoplasm. The GATE Monte Carlo simulation platform was utilized to construct the 160 kV X ray energy spectrum of the RS - 2000 Pro irradiator. In a vacuum environment, the energy deposition processes of single cells and cell populations were simulated, and the dose distributions of the cell nucleus and cytoplasm were computed.Results:In the single cell simulation, the absorbed dose of the cell nucleus was 0.07 Gy, and 0.23 Gy for the cytoplasm. Under the same irradiation duration, the dose of the cell nucleus accounted for approximately 70% of the external irradiation dose. The calculated standard deviations of absorbed dose were 3.03×10?? and 5.73×10?? Gy, respectively, indicating a notable randomness in dose deposition. Since 2 Gy is a widely-adopted dose in radiotherapy fractionation regimens, cell populations were irradiated with 2 Gy. The findings revealed that the internal dose distribution of cell populations exhibited a non-Gaussian distribution, demonstrating the randomness of dose deposition. Specifically, the dose of the cell nucleus was concentrated in the range of 0.6-1.8 Gy, and the dose of the cytoplasm was concentrated in the range of 0.9-2.7 Gy.Conclusions:A curved- surface model of human glioma cells is successfully constructed, which can lay a foundation for improving the accuracy of microscopic dosimetry simulation.
