Effect of hyperbaric oxygen on increasing the sensitivity of non-small cell lung cancer A549 cells to vinorelbine
10.3760/cma.j.cn311847-20210730-00220
- VernacularTitle:高压氧提高非小细胞肺癌A549细胞对长春瑞滨敏感性的研究
- Author:
Hanyu ZHENG
1
;
Yanli LI
1
;
Juju YANG
1
;
Weijian CUI
1
Author Information
1. 商丘市第一人民医院呼吸介入科，商丘 476000
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Vinorelbine;
Lung cancer;
Drug resistance;
Apoptosis
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2022;29(1):57-60,64
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of hyperbaric oxygen（HBO）combined with vinorelbine（NVB）on the sensitivity of non-small cell lung cancer A549 cells，and to explore its mechanism.Methods:After the completion of culture，A549 cells were divided into four groups：blank group，HBO group，NVB group，and HBO+NVB group，with no treatment，0.2 MPa HBO treatment，IC 50 NVB treatment，and 0.2 MPa HBO + IC 50 NVB treatment，respectively. Cell Counting Kit-8（CCK-8）assay was used to detect the cell prolife-ration. Flow cytometry was adopted to detect cell apoptosis. Transwell experiment was used to detect cell migration. And Western blotting was used to detect the expression changes of A549 cell-related proteins. Results:The IC 50 value of NVB on A549 cells was 13.68 μmol/L. The inhibitory rates were 0，4.1%，46.60%，and 68.40% in the blank，HBO，NVB，and HBO+NVB groups，respectively，and the rate in the HBO+NVB group was significantly higher than those in the other three groups（ P<0.05 or P<0.01）. The apoptotic rates were 3.99%，7.63%，36.92%，and 56.49% in the blank，HBO，NVB，and HBO+NVB groups，respectively，and the rate in the HBO+NVB group was significantly higher than those in the other three groups（ P<0.05 or P<0.01）. Compared with the other three groups，the expression level of Bcl-2 in A549 cells in the HBO+NVB group was decreased，while the expression levels of Bad and Caspase-3 were increased（ P<0.01）. The percentages of cells inhibited from migrating in each group were 0，8.90%，42.20%，and 59.40%，respectively，and the HBO+NVB group had the lowest cell migration rate compared with the other three groups（ P<0.05 or P<0.01）. Compared with the other three groups，the expression level of E-Cadherin in the HBO+NVB group was increased，while the expression levels of N-Cadherin，P-glycoprotein（P-gp），and Multidrug resistance protein（MRP）were significantly decreased（ P<0.01）. Conclusion:HBO can increase the sensitivity of A549 cells to NVB by regulating the proteins related to drug resistance，cell migration，and cell apoptosis.
