Effects of hyperbaric oxygen combined with melatonin on migration of lung adenocarcinoma A549 cells and its possible molecular mechanism
10.3760/cma.j.cn311847-20190819-00208
- VernacularTitle:高压氧联合褪黑素对肺腺癌A549细胞迁移的影响及可能的分子机制
- Author:
Yu DAI
1
;
Yuanchang DAI
;
Yinliang QI
Author Information
1. 200333 上海，上海天佑医院
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Lung cancer;
Melatonin
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2021;28(5):573-576
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discuss the effect of hyperbaric oxygen (HBO) combined with melatonin (MLT) on the migration ability of lung adenocarcinoma A549 cells and its possible molecular mechanism.Methods:After the cell culture, lung adenocarcinoma A549 cells were divided into cell control group (control group), hyperbaric oxygen treatment group (HBO group), melatonin treatment group (MLT group), and hyperbaric oxygen combined with melatonin group (MLT+ HBO group). Wound healing assay was used to assess the migration ability of A549 cells, and Western blotting was used to detect the expression of migration-related protein matrix metalloproteinase 9 (MMP-9) in each group.Results:The migration distances of lung adenocarcinoma A549 cells in the MLT group and the MLT+ HBO group after 48 h were shorter than that in the control group, with statistically significant differences ( P<0.01); and the migration distance of lung adenocarcinoma A549 cells in the MLT+ HBO group was reduced even more obvious than that in the MLT group, and the difference was statistically significant ( P<0.01). The expression levels of MMP-9 in the HBO group and the MLT group were lower than that of the control group ( P<0.05); the expression level of MMP-9 in the MLT+ HBO group was even lower than those in the HBO group and the MLT group, and the difference was statistically significant ( P<0.05). Conclusion:Melatonin can inhibit the migration ability of lung adenocarcinoma A549 cells, and hyperbaric oxygen can enhance such inhibitory effect. The mechanism of action may be related to the reduced expression of migration-related protein MMP-9.
