Study on the simulated high load and weightlessness exposure on intervertebral discs imaging and matrix metalloporteinase and its inhibitor expression in New Zealand white rabbits
10.3760/cma.j.cn113854-20211022-00154
- VernacularTitle:模拟高载荷及失重暴露对新西兰大白兔椎间盘影像学与基质金属蛋白酶及其抑制剂表达的相关研究
- Author:
Xuanyu CHEN
1
;
Chao ZHENG
;
Mingkui DU
;
Zhenhua ZHANG
;
Xiang JI
;
Hui ZHAO
;
Kaifeng JIN
;
Weitao ZHONG
;
Liming ZHANG
;
Ji WU
Author Information
1. 首都医科大学电力教学医院骨二科，北京100073
- Publication Type:Journal Article
- Keywords:
Acceleration;
Hypergravity;
Weightlessness simulation;
Rabbits;
Intervertebral disc;
Metalloproteases;
Tissue inhibitor of metalloproteinase-1
- From:
Chinese Journal of Aerospace Medicine
2022;33(1):8-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discuss the influence of high load and weightlessness environment on intervertebral disc by observing and analyzing the imaging changes of the spines in New Zealand white rabbits and the content changes of matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP) in the intervertebral disc under high load and weightlessness environment, and to provide theoretical basis for prevention and delay of lumbar intervertebral disc degeneration under high load and weightlessness environment.Methods:A total of 120 New Zealand white rabbits with balanced age and body weight were randomly and averagely divided into control groups (further grouped by 30 d, 60 d and 90 d) and high load/weightlessness groups (further grouped by 30 d, 60 d and 90 d) with residual single-blind method. High load was simulated by animal centrifuge and weightlessness was simulated by tail suspension. The imaging changes and the positive expression rates of MMP1, MMP3 and TIMP1 in intervertebral disc were detected, and the statistical differences between the high load/weightlessness group and the control group in different time periods were compared, as well as among the different exposure time in load/weightlessness groups.Results:From the imaging observation, the T2-weighted images of lumbar 6 sacral 1 intervertebral disc in experimental animals decreased to some extent. The positive rates of MMP1 and MMP3 in each high load/weightlessness group were higher than those in the control group for the same exposure time, and the differences were statistically significant ( t=22.97-145.51,all P<0.001). The positive rates of MMP1 and MMP3 increased with the extension of exposure time, and there were statistically differences among the groups with different exposure time in high load/weightlessness group ( F=2531.10, 1758.80, both P<0.001). The positive rate of TIMP1 in each high load/weightlessness group was higher than that in the control group for the same exposure time, and the difference was statistically significant ( t=29.34-65.05, all P<0.001). There was statistically difference on TIMP1 positive rate among different load/weightlessness exposure time groups ( F=462.20, P<0.001). Conclusions:High load/weightlessness can cause the MMP1, MMP3 and TIMP1 content changes in animal lumbar disc. The changes of TIMP1 are obvious in the early stage and the sensitivity is decreased in the late stage. High load/weightlessness may lead to changes in the contents of MMP and TIMP in intervertebral discs, which may accelerate intervertebral disc degeneration.
