Establishment of an animal model of button battery-induced esophageal injury ex vivo and investigation of interventive effect of vegetable oil
10.3760/cma.j.cn321463-20241226-00203
- VernacularTitle:离体纽扣电池致食管腐蚀伤动物模型构建及植物油的干预影响
- Author:
Zhaofei LI
1
;
Dean ZHAO
;
Mingyang LI
;
Lingchao WANG
;
Weiwei MA
Author Information
1. 新乡医学院第一附属医院儿科，新乡 453100
- Publication Type:Journal Article
- Keywords:
Esophageal diseases;
Corrosion;
Plant oils;
Rabbits
- From:
Chinese Journal of Digestive Endoscopy
2025;42(10):817-822
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an ex vivo model of button battery-induced esophageal injury in New Zealand rabbits and evaluate the interventive effects of vegetable oil through esophageal histopathological scoring.Methods:Thirty-six esophageal segments (≈5 cm length) from 10 cm specimen of 18 male rabbits were divided into model groups (15-min, 2-hr, and 6-hr exposure; n=6/group) and intervention groups (olive/peanut/soybean oil infusion; n=6/group). Button batteries were inserted to esophageal segments of model groups. Voltage drop of the battery, pH at negative electrode contact site, and histopathological injury scores were assessed. In the intervention group, button batteries were placed in the esophageal segment for 15 minutes, and olive oil, peanut oil, and soybean oil were infused. The above indicators were observed 6 hours after the button batteries were placed. One-way ANOVA was used to compare the differences of esophageal mucosal tissue damage across time points and oil types. Results:There was no significant difference in the pH value of the negative electrode contact area (9.50±0.56, 10.67±0.80, 11.17±0.40, F=1.955, P>0.05), but the discharge voltage (42.67±4.60 mV, 90.00±2.07 mV, 125.83±2.80 mV, F=156.9, P<0.001) and pathological injury scores (3.50±1.09 scores, 5.33±0.72 scores, 8.67±0.67 scores, F=9.623, P=0.002) in the model groups were significantly different. There was significant difference in pathological injury score between the 6-hour model group and the three intervention groups (8.67±0.67 scores, 7.33±0.62 scores, 6.50±0.43 scores, 6.67±0.42 scores, F=3.279, P=0.042). The difference in pathological injury score between the peanut oil intervention group and the 6-hour model group was statistically significant (mean difference=2.167, P<0.05). Conclusion:This ex vivo model effectively simulates battery-induced esophageal injury. Household peanut oil demonstrates significant protective effects, providing experimental basis for prehospital management of battery corrosion.
