Study on the mitochondrial mass of peripheral blood immune cells in patients with schizophrenia
10.3760/cma.j.cn113661-20220322-00068
- VernacularTitle:精神分裂症患者外周血免疫细胞线粒体质量变化的研究
- Author:
Tianyi ZHAO
1
;
Shize LIN
;
Yichuan LIN
;
Xiaofeng HUANG
;
Jinyi WANG
Author Information
1. 福建医科大学第三临床医学院，福州 350122
- Publication Type:Journal Article
- Keywords:
Schizophrenia;
T-Lymphocytes;
Mitochondria
- From:
Chinese Journal of Psychiatry
2022;55(4):254-262
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the differences in the variation characteristics of peripheral blood immune cell subsets and their mitochondrial mass between schizophrenia patients (SZ) and healthy individuals (HC).Methods:The selected subjects included 487 schizophrenic patients aged 20 to 70 (schizophrenia group), who were diagnosed and treated in Quanzhou Third Hospital from February 2021 to October 2021, and 175 healthy individuals (control group) of the same age and during the same period. Their age, gender, years of education, and years of illness were collected. The psychopathological symptoms of the patients were assessed by PANSS (Positive and Negative Syndrome Scale). The flow cytometry was used to analyze the percentage, the absolute count of T cells and their subsets in peripheral blood, the mitochondria mass (MM) and the variation in the single-cell mitochondria mass (SCMM) of these T cells. To determine whether the mitochondrial mass parameters were consistent with clinical manifestations, the test data collected from 32 first diagnosed patients were compared before and after treatment. The analysis methods involved in the study mainly include the Human Lymphocyte Mitochondrial Function Analysis System (HLMFAS) used to calibrate of the mitochondrial test results, the SPSS software used for statistical analysis, and the flowjo software used for the t-SNE dimension reduction analysis of the flow cytometry data.Results:The SCMM value of CD8 +T cells (abbreviated as CD8 +) in SZ was significantly higher than those values in HC ( P<0.001). The threshold of the ROC curve of the SCMM(CD8 +) between SZ and HC was greater than 95.84. SZ showed a significantly higher SCMM(CD8 +) value than HC across all age groups (20-70 years). For the 32 first diagnosed patients, the test data were monitored before and after treatment. The results indicated a positive linear correlation between the SCMM of CD8 + and the PNASS data ( P<0.05,ρ (Spearman)>0). Conclusion:Schizophrenic patients have abnormal mitochondrial metabolism in their peripheral immune cells with an up-regulated SCMM, indicating possible damage to the mitochondria.
