Study of Jiedu Huayu granules on antagonizing intestinal endotoxemia in liver failure by improving inflammation and intestinal barrier damage
10.3760/cma.j.cn501113-20240907-00482
- VernacularTitle:解毒化瘀颗粒通过改善炎症和肠屏障损伤拮抗肝衰竭肠源性内毒素血症的研究
- Author:
Tingshuai WANG
1
;
Riyun ZHANG
;
Chengyu YA
;
Dewen MAO
;
Rongzhen ZHANG
Author Information
1. 广西中医药大学第一附属医院肝病一区，南宁 530000
- Publication Type:Journal Article
- Keywords:
Liver failure;
Endotoxemia;
Intestinal barrier;
Jiedu Huayu granules;
Therapeutic
- From:
Chinese Journal of Hepatology
2025;33(9):872-879
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mechanistic role of Jiedu Huayu granule in improving intestinal mucosal barrier functional damage and protecting against liver failure in mice induced by D-galactosamine combined with lipopolysaccharide.Methods:Fifty mice were randomly divided into a normal group, a model group, a low-dose Jiedu Huayu granule group (4.16 g·kg -1·d -1), a medium-dose Jiedu Huayu granule group (8.32 g·kg -1·d -1), and a high-dose Jiedu Huayu granule group (16.64 g·kg -1·d -1). D-galactosamine combined with lipopolysaccharide was administered once to establish an acute liver failure mouse model, followed by corresponding drug intervention. Retro-orbital blood samples were collected to measure serum alanine aminotransferase, aspartate aminotransferase, and total bilirubin levels six hours after modeling in mice. The endotoxin content was detected using the limulus reagent method. The content of serum interleukin-1β, tumor necrosis factor-α, and interferon-γ levels were detected using enzyme-linked immunosorbent assay. Hematoxylin-eosin staining was used to observe liver and intestinal pathological tissues.Western blot and RT-PCR were used to determine the protein and mRNA expression of Occludin, Zo-1, Tricellulin, and CLDN3 in intestinal tissues. Multiple quantitative data were analyzed using one-way ANOVA, following a normal distribution. LSD-t test was used for statistical analysis between inter-groups. Results:The medium-and high-dose Jiedu Huayu granules had reduced and improved liver function impairment, liver pathological tissue damage, intestinal barrier damage, serum endotoxin and inflammatory factors ( P<0.05), and increased intestinal expression of occludin, Zo-1, Tricellulin, and CLDN3 ( P<0.05) in mice group with acute liver failure. Conclusion:Jiedu Huayu granules can reduce liver inflammatory damage, gut-derived endotoxemia, bacterial endotoxins entering the liver, and protect intestinal barrier function, thereby remissioning liver inflammation and antagonizing liver failure in mice with acute liver failure.
