Composition and diversity of facial microbial communities in patients with moderate acne
10.3760/cma.j.cn114657-20241025-00150
- VernacularTitle:中度痤疮患者面部微生物群落结构组成和多样性特征分析
- Author:
Yuanyuan XIAO
1
;
Shangke LIU
;
Xinyu LIN
;
Yilin DU
;
Qinchuan BAI
;
Haixia KUANG
;
Chunyu HUANG
Author Information
1. 西南医科大学附属医院皮肤科，泸州 646000
- Publication Type:Journal Article
- Keywords:
Acne vulgaris;
High-throughput sequencing;
Microbial communities
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2025;31(4):376-381
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the composition and diversity characteristics of facial microbial communities in patients with moderate acne.Methods:This prospective study enrolled 30 patients with moderate acne [12 males, 18 females; aged 21-30 (25.4±2.5) years] from the Department of Dermatology, Sichuan Provincial People′s Hospital from March to July 2021. Thirty healthy controls [13 males, 17 females; aged 24-29 (25.2±1.4) years] were included during the same period. Facial skin swabs were collected from both groups. Total DNA was extracted, followed by PCR amplification, library preparation, and PE250 sequencing. After splicing, filtering, denoising, and chimera removal, amplicon sequence variants (ASV) feature tables and representative sequences were generated to compare microbial community differences between the two groups.Results:A total of 60 samples were sequenced, yielding 2 021 342 valid sequences. The 16S rRNA gene sequences were clustered into 8 379 ASV, with 589 ASV shared between the two groups, while 6 445 ASV were uniquely identified in healthy controls. At the phylum level, both groups showed similar dominant phyla: Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. At the genus level, the acne group was predominantly colonized by Ralstonia (relative abundance 31.85%) and Staphylococcus (28.04%), while healthy controls exhibited more balanced distributions, primarily Staphylococcus (9.18%) and Enhydrobacter (7.37%). Alpha diversity analysis, Beta diversity analysis, and LefSe analysis revealed statistically significant differences in microbial communities between groups ( R2=0.157, P<0.001). The acne group showed lower microbial richness and evenness compared to healthy controls (both P<0.001). Conclusion:Patients with moderate acne exhibit microecological imbalance in facial microbial communities, characterized by reduced microbial richness and evenness.
