Acoustically sensitive oxygen-carrying nanodroplets boost cardiac patch function and promote myocardial infarction repair
10.3760/cma.j.cn131148-20250121-00034
- VernacularTitle:声敏型载氧纳泡提高心肌补片活性促进心肌梗死修复
- Author:
Hao WANG
1
;
Yuxin GUO
1
;
Yueying CHEN
1
;
Wendi SU
1
;
Yugang HU
1
;
Qing ZHOU
1
Author Information
1. 武汉大学人民医院超声影像科，武汉 430060
- Publication Type:Journal Article
- Keywords:
Low-intensity pulsed ultrasound;
Nano phase-change microbubbles;
Nano-oxygen carriers;
Cardiac patch;
Hydrogel
- From:
Chinese Journal of Ultrasonography
2025;34(8):645-653
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct an ultrasound-responsive nano-oxygen carrier，and to enhance cell survival within myocardial patches and promote myocardial infarction（MI）repair.Methods:Ultrasound-responsive phase-change nanobubbles（ND）were first prepared and integrated into GelMA hydrogel to construct myocardial patches. Low-intensity pulsed ultrasound（LIPUS）irradiation was applied to explore whether the nanobubbles could optimize the hydrogel properties. Hemoglobin（Hb）was further encapsulated into the nanobubbles to construct an oxygen carrier（ND-Hb）. In vitro and in vivo experiments were conducted to evaluate whether the optimized myocardial patches could improve cell survival and facilitate MI repair. In vitro，cell-loaded patches were divided into 6 groups（control，ND，Hb，LIPUS，LIPUS+ND，and LIPUS+oxygen carrier groups）to assess the cell viability and protein expression. In vivo，an acute MI model was established in SD rats，which were randomly assigned to 4 groups（control，Hb，LIPUS+ND，and LIPUS+oxygen carrier groups）.Myocardial patches were implanted，and cardiac function（echocardiography），cell survival（BLI imaging），angiogenesis（CD31 and α-SMA immunofluorescence）and connexin protein expression（Cx43）were evaluated. Results:Following the incorporation of ND and LIPUS irradiation，scanning electron microscopy revealed numerous micropores（about 2 μm）within the hydrogel were observed by scanning electron microscopy. The nano-oxygen carrier was successfully constructed，with a particle size of（301.2 ± 92.4）nm，and released oxygen under LIPUS stimulation. In vitro，at days 3，7，and 14，the cell survival rates in the LIPUS+oxygen carrier group［（89.6 ± 2.1）%，（79.3 ± 1.8）%，（70.9 ± Conclusions:This study successfully employs LIPUS combined with ND-Hb to enhance hydrogel properties，facilitating nutrient exchange within myocardial patches. Additionally，ultrasound-mediated oxygen release improves seed cell survival and promotes myocardial infarction repair.
