The application and prospect of CRISPR/Cas in monkeypox virus detection
10.3760/cma.j.cn114452-20250630-00382
- VernacularTitle:CRISPR/Cas技术在猴痘病毒检测中的应用与展望
- Author:
Yingwei CHEN
1
;
Xueliang WANG
Author Information
1. 上海市临床检验中心 质控品研发实验室，上海200126
- Publication Type:Journal Article
- Keywords:
Nucleic acid testing;
Monkeypox virus;
CRISPR/Cas;
Biosensor;
Point-of-Care Testing
- From:
Chinese Journal of Laboratory Medicine
2025;48(10):1264-1270
- CountryChina
- Language:Chinese
-
Abstract:
The monkeypox pandemic has caused two public health emergencies of international concern from 2022 to 2025,causing great impact on the global health system. Early diagnosis and treatment of the monkeypox virus depend on quick, sensitive, and accurate detection methods. The clustered regular interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system, characterized by its unique target gene sequence recognition, and cis-and trans-cleavage activity, has been regarded as the next-generation nucleic acid detection tool in the field of pathogen diagnosis. CRISPR/Cas has been used for detecting monkeypox virus in combination with different amplification and result readout approaches. Although it still faces many challenges, the CRISPR/Cas system is expected to achieve diversified development and provide strong technical support for rapid, accurate, and affordable detection approaches for infectious diseases.
