Establishment of LC-MS/MS method for detecting plasma Lyso-GL-3 concentration
10.3760/cma.j.cn114452-20241031-00599
- VernacularTitle:LC-MS/MS检测血浆Lyso-GL-3浓度的方法学建立
- Author:
Bing DAI
1
;
Jie QIU
1
;
Yani DENG
1
;
Chunyan TANG
1
;
Jieqiong SU
1
;
Qi YANG
1
;
Dan GUO
1
;
Yan YU
1
Author Information
1. 长沙金域医学检验实验室有限公司，长沙 410005
- Publication Type:Journal Article
- Keywords:
Fabry disease;
Liquid chromatography tandem mass spectrometry;
Globotriaosylsphingosine;
Plasma
- From:
Chinese Journal of Laboratory Medicine
2025;48(8):1041-1047
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for accurate determination of plasma Lyso-GL-3 concentration.Method:Solid phase extraction technology was used to process plasma samples, and under positive ion mode and multiple reaction monitoring (MRM) conditions, LC-MS/MS was used to determine the concentration of Lyso-GL-3. The linear range, detection and quantification limits, accuracy, precision, matrix effect, carrier effect of the method, and plasma sample stability were validated. And the accuracy of Lyso-GL-3 positive patients was compared by combining enzymatic and genetic testing results.Result:Lyso-GL-3 had good linearity in the range of 1.25-400 nmol/L. The detection limit and quantification limit were 0.15 nmol/L and 0.50 nmol/L, respectively. The spiked recovery rate was 88.78%-108.96%. The coefficient of variation ( CV) for intra batch precision, inter batch precision, and matrix effect were all less than 15%, the result of carrier effect was 0.55%. Plasma samples could be stably stored for 30 days under refrigeration conditions. The clinical conformity of the patient was 100%. Conclusion:The established LC-MS/MS detection method for plasma Lyso-GL-3 concentration takes 2.5 minutes, which is simple, fast, accurate, and reliable.
