Clinical research of Eccentric mixing in correcting EDTA-dependent pseudothrombocytopenia
10.3760/cma.j.cn114452-20250206-00060
- VernacularTitle:偏心混匀法纠正乙二胺四乙酸依赖性假性血小板减少症的临床研究
- Author:
Dan LIU
1
;
Guangming SU
1
;
Menghan YANG
1
;
Wei YANG
1
;
Xiuru GUAN
1
Author Information
1. 哈尔滨医科大学附属第一医院检验科，哈尔滨150001
- Publication Type:Journal Article
- Keywords:
Blood Platelets;
Mixing;
Ethylenediaminetetraacetic acid;
EDTA-dependent pseudothrombocytopenia
- From:
Chinese Journal of Laboratory Medicine
2025;48(5):597-602
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Evaluate To evaluate the efficacy of eccentric mixing in correcting EDTA-dependent pseudothrombocytopenia (EDTA-PTCP) and platelet (PLT) count.Methods:A case-control study was conducted using EDTA-PTCP patient samples collected from the First Affiliated Hospital of Harbin Medical University from April 1st, 2024, to December 27th, 2024, including 82 cases with mean age 57.98±17.28 years old. EDTA-PTCP was defined as PLT≥3 under microscopy. Samples underwent eccentric mixing at 500 r/min or 3000 r/min for durations ranging from 0.5 to 13 minutes to determine the optimal PLT disaggregation protocol. Repeated-measures ANOVA and paired t-tests were used to analyze PLT results. While chi-square tests were used to compare effective disaggregation rates under the optimal eccentric mixing method and sodium citrate anticoagulation. Statistical significance was defined as P<0.05. Results:Eccentric mixing at 3000 r/min for 10 minutes significantly elevated PLT counts to (135.6±63.91)×10 9/L, compared with untreated group (47.72±25.47)×10 9/L ( t=5.74, P<0.001). Blood analyzer showed that 3000 r/min for 10 minutes method did not alter red blood cell (4.32±0.76 vs. 4.27±0.75)×10 12/L nor white blood cell (6.17±2.22 vs. 6.36±2.14)×10 9/L counts compared with untreated group, in which red blood cell (4.27±0.75)×10 12/L ( t=0.40, P=0.690) and white blood cell(6.36±2.14)×10 9/L ( t=0.48, P=0.630). The eccentric mixing achieved a disaggregation rate of 92.68% while sodium citrate anticoagulation 63.41%, with significant differenct (χ2=20.5, P<0.001). Conclusion:Eccentric mixing at 3000 r/min for 10 minutes effectively disaggregates platelets in EDTA-PTCP, correcting PLT counts without affecting other blood cells. This technique outperforms anticoagulant replacement strategies, offering a more efficient solution for clinical use as an alternative for changing anticoagulant.
