Establishment and evaluation of a new large animal model for fetal cardiopulmonary bypass
10.3760/cma.j.cn112434-20250309-00073-1
- VernacularTitle:一种新型胎儿体外循环实验大动物模型的建立与评价研究
- Author:
Kaiyu WANG
1
;
Qingqing MENG
;
Dasheng NING
;
Chunfeng ZHU
;
Jinjin YU
;
Jimei CHEN
;
Jian ZHUANG
;
Haiyun YUAN
Author Information
1. 广东省医学科学院 广东省心血管病研究所 广东省人民医院心外科，广州 510800
- Publication Type:Journal Article
- Keywords:
Fetal Cardiopulmonary Bypass;
Cardiac surgery;
Animal Experiments;
Model Validation;
Evaluation study
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2025;41(10):599-605
- CountryChina
- Language:Chinese
-
Abstract:
Objective:In order to reduce the trauma associated with fetal cardiopulmonary bypass(F-CPB), Our team plans to develop a minimally invasive F-CPB through a small incision in the right axilla. The efficacy of this technique will be verified by using a big experimental animal model, thereby laying the foundation for fetal cardiac surgery supported by F-CPB in the future.Methods:Ten pregnant sheep were divided into F-CPB group(n=5) and control group(n=5). After fasting for 24 h, fetal lambs in the F-CPB group underwent a right axillary incision to establish F-CPB running for 1 h; The control group of fetal lambs only expose heart 1 h without F-CPB. Collect blood sample for laboratory test at the CPB vehicle before(T0), 30 min(T1), and 1 h after F-CPB running(T2) for the F-CPB group and through Superior Vena Cava before(T0), 30 min(T1), and 1 h after F-CPB running(T2) for the control group.Results:The blood routine indicators such as RBC, HCT, and Hb in the F-CPB group of fetal lambs decreased significantly during F-CPB, and their distribution showed significant statistical differences compared with the control group( P<0.05). There were no significant statistical differences in blood gas indicators such as pH, PO 2, PCO 2, and lactate concentration between the F-CPB group and the control group( P>0.05). There was no statistically significant difference in the concentration of cTnI in fetal lamb serum at each time point( P>0.05). There were significant statistical differences( P<0.05) in the distribution of fetal lamb Alb, γ-GGT, CK concentration and cholesterol concentration at various time points in the F-CPB group compared with the control group in liver function examination. In addition, the distribution of BUN in fetal lambs showed a significant difference between the two groups( P=0.006). Conclusion:A minimally invasive F-CPB via small incision in the right axilla is safe and feasible. The experimental animal model has demonstrated that this technique has minimal impact on the vital organ functions and internal environment of fetal lambs, thereby laying the foundation for clinical fetal cardiac surgery in the future.
