Advances in Cas12/13 protein trans-cleavage activity for point-of-care testing
10.3760/cma.j.cn114452-20240614-00311
- VernacularTitle:基于Cas12/13蛋白的反式切割活性在即时检测中的研究进展
- Author:
Yaozhou WU
1
;
Yingying SUN
;
Yanbin CHANG
;
Keke LI
;
Lianhua WEI
Author Information
1. 兰州大学第一临床医学院2022级临床检验诊断学专业，兰州 730000
- Publication Type:Journal Article
- Keywords:
CRISPR/Cas system;
Point-of-care testing;
Colorimetry;
Microfluidic analytical techniques;
Smartphones;
Diagnostic equipment
- From:
Chinese Journal of Laboratory Medicine
2025;48(2):303-308
- CountryChina
- Language:Chinese
-
Abstract:
The clustered regularly interspaced short palindromic repeat-CRISPR-associated protein (CRISPR-Cas) system is renowned for its exceptional gene-editing capabilities. In recent years, the discovery of the trans-cleavage activity of Cas12 and Cas13 proteins has shown great potential in the field of molecular diagnostics. The trans-cleavage activity of the CRISPR-Cas system has been widely applied in point-of-care testing (POCT). This article will provide a detailed discussion on the principles, advantages, and challenges of combining the CRISPR-Cas system with lateral flow assays, colorimetric methods, microfluidic technologies, smartphone applications, and portable detection sets. The CRISPR-Cas system demonstrates versatility in the POCT field with its ability to detect nucleic acids, pathogenic microorganisms, and non-nucleic acid targets. As CRISPR-Cas-based POCT continues to advance, these developments provide strong support for the formulation of personalized medical and public health strategies, further promoting the realization of precision medicine.
