Pathogenic characteristics and phylogenetic analysis of a norovirus GⅡ.4P16 outbreak in Shenzhen
10.3760/cma.j.cn114452-20241122-00642
- VernacularTitle:深圳市一起GⅡ.4［P16］型诺如病毒暴发疫情的病原特征和进化分析
- Author:
Lilan ZENG
1
;
Yi HAN
1
;
Haobin MA
1
;
Ying WANG
1
;
Yuan HUANG
1
;
Yanping MA
1
Author Information
1. 深圳市南山区疾病预防控制中心（深圳市南山区卫生监督所），深圳 518054
- Publication Type:Journal Article
- Keywords:
Norovirus;
Genotype;
Phylogenetic
- From:
Chinese Journal of Laboratory Medicine
2025;48(2):276-279
- CountryChina
- Language:Chinese
-
Abstract:
An 87-year-old female patient went to a hospital in Nanshan District on July 18, 2023 because of "vomiting and diarrhea for 2 days". She lived in a nursing home, where 43 similar cases were found within 8 days, including 31 elderly people and 12 caregivers. On July 23, 2023, 13 anal swab samples of the elderly patients were collected, to further find the source of infection, 89 anal swab samples of related nursing assistants and 9 environmental swab samples were collected at the same time. The pathogen was identified by reverse transcription polymerase chain reaction, a total of 22 out of 102 anal swab specimens were positive for Norovirus GⅡ, and the positive rate was 12/13 in the elderly people and 11.23% (10/89) in the caregivers, and one norovirus GⅡ positive sample among 9 environmental smear samples. The polymerase region and partial gene sequences of the capsid VP1 region were amplified and sequenced for the Norovirus-positive specimens, among which 20 strains were successfully sequenced and showed GⅡ.4[P16] under the Norovirus online typing tool. The sequences of 11 elderly people were highly homologous to those of 9 caregivers, with a homology of 95% in the polymerase region and 99% in the capsid VP1 region, which had sequence homology of 99% compared with the Genbank reference sequences of OP009779 (2022 Chengdu strain), OQ930719 (2023 Nanjing strain), and OL336359 (2021 Beijing strain).
