Application of T-lymphocyte detailed subsets immunosurveillance in B-lymphoproliferative disorder
10.3760/cma.j.cn114452-20240815-00456
- VernacularTitle:T细胞精细亚群免疫监测在B淋巴细胞增殖性疾病中的应用
- Author:
Ruifeng TIAN
1
;
Xiaoya DONG
1
;
Fei LU
1
;
Guosheng LI
1
Author Information
1. 山东大学齐鲁医院血液科，济南 250012
- Publication Type:Journal Article
- Keywords:
Lymphoproliferative disorders;
B-lymphoproliferative disorder;
CD4 +T cell;
CD8 +T cell;
Regulatory T cell;
Flow cytometry
- From:
Chinese Journal of Laboratory Medicine
2024;47(12):1426-1434
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the difference in the proportions of T-lymphocyte subsets and the immunological monitoring profiles between patients with B-lymphoproliferative disorder(B-LPD) and healthy individuals.Methods:This clinical observational study involved 194 B-LPD patients [122 males, 72 females, average age (64±20)] treated in Qilu Hospital of Shandong University from May 14th, 2022 to January 19th, 2024. The patient cohort included 76 cases of chronic lymphocytic leukemia (CLL), 23 cases of diffuse large B-cell lymphoma (DLBCL), 17 cases of follicular lymphoma (FL), 12 cases of hair-cell leukemia (HCL), 10 cases of lymphoplasmacytic lymphoma/Waldenstr?m macroglobulinemia (LPL/WM), 26 cases of mantle cell lymphoma (MCL), 20 cases of marginal zone lymphoma (MZL), and 10 cases of unclassified CD5 -B lymphocytic proliferative disease unclassified (B-LPD-U). At the same time, 45 health control (HC) samples [22 males, 23 females, aged 62(±17)] from the medical examination center of our hospital in November 18th, 2023 were collected. The T cell subsets were meticulously analyzed using flow cytometry, including CD4 +naive T cell [CD4 +TN (CD3 +CD4 +CD45RA +CD62L +) ], CD4 +central memory T cell [CD4 +TCM (CD3 +CD4 +CD45RA -CD62L +) ], CD4 +terminally differentiated effector memory cells expressing CD45RA [CD4 +TEMRA (CD3 +CD4 +CD45RA +CD62L -) ], CD4 +effector memory T cell [CD4 +TEM (CD3 +CD4 +CD45RA -CD62L -) ], CD8 +TN (CD3 +CD8 +CD45RA +CD62L +), CD8 +TCM (CD3 +CD8 +CD45RA -CD62L +), CD8 +TEMRA (CD3 +CD8 +CD45RA +CD62L -), CD8 +TEM (CD3 +CD8 +CD45RA -CD62L -), naive regulatory T cell [nTreg (CD3 +CD4 +CD127 dim+CD25 +CD45RA +) ], memory Treg [mTreg (CD3 +CD4 +CD127 dim+CD25 +CD45RA -) ] and activated Treg [aTreg (CD3 +CD4 +CD127 dim+CD25 +HLA-DR +) ]. The difference of T cell subsets data were compared by Mann-Whitney test. Result:The proportionof CD3 +T cells within lymphocyte in B-LPD groups (CCL group 13.15%, DLBCL group 41.75%, FL group 30.52%, HCL group 34.24%, LPL/WM group 40.58%, MCL group 20.67%, MZL group 26.36%, CD5 -B-LPD-U group 17.49%) was significantly lower than HC group (64.85%). The percentage of CD4 +T cells in B-LPD groups (CCL group 46.63%, DLBCL group 40.76%, FL group 42.77%, HCL group 43.81%, LPL/WM group 43.02%, MCL group 45.58%, MZL group 43.95%, CD5 -B-LPD-U group 46.91%) was also significantly lower than HC group (54.61%). Conclusions:B-LPD diseases impair T cell immune function. The increased presence of CD4 +TEM, the reduced level of CD8 +TEMRA and the elevated aTreg suggest that B-LPD may suppress CD8 +T cell-mediated cytotoxicity and enhance the immunosuppressive activity of Treg.
