Characteristics of drug resistance and molecular transmission networks among preoperative HIV/AIDS patients in Ningxia from 2018 to 2023
10.3760/cma.j.cn112866-20241226-00187
- VernacularTitle:宁夏2018—2023年术前HIV/AIDS患者耐药及分子传播网络特征探析
- Author:
Xiaohong ZHU
1
;
Lihua ZHAO
;
Zhonglan WU
;
Jianxin PEI
;
Yufeng LI
;
Yichang LIU
;
Xiaofa MA
;
Ling SONG
Author Information
1. 宁夏医科大学 公共卫生学院，银川 750004
- Publication Type:Journal Article
- Keywords:
HIV-1;
Preoperative detection;
Drug resistance characteristics;
Molecular transmission network
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(3):287-293
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aimed to analyze the genetic subtypes and drug resistance transmission characteristics of HIV-1 among the preoperative population in Ningxia from 2018 to 2023, to provide a scientific basis for the prevention and control of the AIDS epidemic.Methods:Plasma samples and demographic information of HIV/AIDS patients receiving antiviral treatment in Ningxia from 2018 to 2023 were collected. Blood samples with a viral loads >200 copies/ml from preoperative testing were amplified, sequenced, and subjected to genotypic resistance testing to analyze their genetic subtypes and drug resistance characteristics. The TN93 model in MEGA11 software was used to calculate the genetic distance between each pair of all sequences, and a molecular transmission network was constructed in Cytoscape 3.10.0 with 1.9% as the genetic threshold.Results:Among 101 preoperative HIV/AIDS patients, CRF07_BC and CRF01_AE were the predominant subtypes. The majority were male (85.15%, 86/101), aged 41-60 years (45.54%, 46/101), residing in Yinchuan city (61.39%, 62/101), and infected via heterosexual transmission (71.29%, 72/101), with most cases being late-detected. Of 39 drug-resistant sequences, resistance to non-nucleoside reverse transcriptase inhibitors (NNRTIs) alone (18.81%, 19/101) and dual resistance to nucleoside reverse transcriptase inhibitors (NRTIs)-NNRTIs (13.86%, 14/101) were most common. Among 44 sequences forming 13 transmission clusters, nine clusters harbored drug-resistant mutations. Four subtypes entered the molecular network, primarily involving heterosexual transmission, individuals with junior high school education or below, and men aged≥50 years.Conclusions:From 2018 to 2023, the preoperative HIV/AIDS patients had diversified genetic subtypes, with higher rates of overall drug resistance and late detection, stronger drug resistance and higher mortality rate. Strengthening molecular epidemiological research and developing targeted screening strategies are critical to improve early detection and reduce transmission risks.
