Molecular epidemiology of norovirus among diarrheal patients in Linyi, 2021 to 2023
10.3760/cma.j.cn112866-20241114-00163
- VernacularTitle:2021—2023年临沂市腹泻患者中诺如病毒分子流行病学研究
- Author:
Chengwei LI
1
;
Jing WANG
1
;
Xiangliang LIU
1
;
Deqing YIN
1
;
Shengxiang JI
1
;
Qianqian DUAN
1
Author Information
1. 临沂市疾病预防控制中心，临沂 276001
- Publication Type:Journal Article
- Keywords:
Norovirus;
Epidemiology;
Genotypes
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(2):202-207
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the molecular epidemiological characteristics of norovirus (NoV) among infectious diarrhea patients in Linyi from 2021 to 2023, and provide the scientific evidence for norovirus control and prevention.Methods:The epidemiological information and fecal samples of patients with infectious diarrhea in Linyi from 2021 to 2023 were collected and detected by real-time fluorescent quantitative PCR. The RdRp and capsid gene VP1 region of norovirus-positive specimens were amplified and sequenced used to analyze their genotype, phylogeny and homology.Results:Among the 2 311specimens, 123 (5.32%) were positive of NoV, with GI/GII group infection accounted for 17.07% (21/123) and 77.24% (95/123), respectively, GI/GII group mixed infection accounted for 5.69% (7/123). The detection rate of <1 year age group was the highest (8.86%), followed by the 1-3 years group (7.11%, 30/422) and the over 60 years group (5.29%, 23/123). The highest NoV detection rate was 7.96% (45/565) in the urban area, and the most abundant genotypes were found in the northern mountainous area. Sequence analysis showed that 61 identified NoV strains could be divided into 12 genotypes, with 4 NoV GI and 8 NoV GII. The dominant genotype differed during the 3 years, with GII.4 Sydney [P16] in 2021, GII.4 Sydney [P31] in 2022, and GII.2[P16] in 2023.Conclusions:NoV GII group was the main NoV infection in Linyi from 2021-2023 and genotypes were diverse. Children under 3 years of age and elderly people over 60 years old as susceptible groups should be given special protection. In addition, the monitoring of NoV genotype diversification should be strengthened in northern mountainous areas.
