Isolation and identification of mosquito-borne viruses in Huachuan county and Huanan county, Heilongjiang province, China
10.3760/cma.j.cn112866-20241223-00184
- VernacularTitle:黑龙江省桦川县和桦南县蚊媒病毒的分离与鉴定
- Author:
Han CHEN
1
;
Fengming LIU
;
Liqin YU
;
Fan LI
;
Shihong FU
;
Qikai YIN
;
Qianqian CUI
;
Ruichen WANG
;
Kai NIE
;
Mingjia BAO
;
Huanyu WANG
;
Songtao XU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 传染病溯源预警与智能决策全国重点实验室，北京 102206
- Publication Type:Journal Article
- Keywords:
Mosquito-borne viruses;
Culex flavivirus;
Menghai rhabdovirus;
Nam Dinh virus
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(2):182-188
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mosquito-borne viruses carried by mosquito specimens collected from Huachuan county and Huanan county in Heilongjiang province.Methods:Mosquito samples were collected locally in 2023 and processed in the laboratory. Homogenates of the mosquitoes were inoculated into cells for virus isolation, followed by molecular and bioinformatics analyses of the viral isolates.Results:In 2023, ten viral isolates were obtained from Anopheles sinensis specimens collected in Heilongjiang province, China. Among these isolates, one was identified as Culex flavivirus (CxFV), one as Menghai rhabdovirus (MRV), and eight as Nam Dinh virus (NDiV). The phylogenetic analysis showed that CxFV belongs to genotype I and is clustered with the strains isolated from Liaoning province in 2011 and Ningxia Hui autonomous Region in 2019 in the same evolutionary branch, with amino acid similarity ranging from 98.2% to 99.2% and nucleotide similarity ranging from 98.8% to 99.2%. The MRV strain belongs to the same evolutionary subclade as the strain detected in Guangdong, with both nucleotide and amino acid similarity of 98.0%. Eight NDiV isolates clustered with the South Korean isolates on the same evolutionary branch, forming an independent evolutionary sub-branch. The nucleotide similarity among these eight isolates ranged from 98.5% to 99.7%, while the amino acid similarity ranged from 98.1% to 99.7%. In comparison, when matched with other NDiV isolates from China, the nucleotide similarity of these eight isolates ranged from 94.1% to 97.8%, and the amino acid similarity ranged from 93.5% to 97.7%.Conclusions:This study represents the first isolation of CxFV, MRV, and NDiV in Heilongjiang province, China, and the findings provide fundamental data for the prevention and control of mosquito-borne viral diseases in this region.
