Analysis of the whole genome characteristics of influenza A(H1N1)pdm09 subtype in Wuxi city, 2018-2023
10.3760/cma.j.cn112866-20250213-00029
- VernacularTitle:2018—2023年无锡市甲型H1N1流感病毒全基因组特征分析
- Author:
Guangyuan MA
1
;
Xiaoyu DING
;
Jing BAO
;
Yong XIAO
;
Qi ZHOU
;
Chun′an YU
Author Information
1. 无锡市疾病预防控制中心(南京医科大学附属无锡疾病预防控制中心)，无锡 214023
- Publication Type:Journal Article
- Keywords:
A(H1N1)pdm09;
Whole genome sequencing;
Variation;
Glycosylation
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(3):353-360
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the evolution and variation characteristics of the H1N1 influenza virus in Wuxi City from 2018 to 2023.Methods:Real time PCR was used to perform nucleic acid testing on throat swab samples of influenza like cases sent to sentinel hospitals for testing. The influenza A (H1N1) pdm09 positive samples were subjected to cell culture, and nucleic acid was extracted from strains with a red blood cell agglutination test (HA) ≥1∶8. The whole genome sequence was amplified, and a library was constructed. The MiSeq sequencer was used for sequencing on the machine. Using NC_026431.1 as a reference strain, we analyzed the offline data using CLC Genomics Workbench (Version 23) software. MEGA 7.0 software was used to construct a phylogenetic tree, and NetNGlyc 1.0 Server software was used to predict N-glycosylation sites.Results:The nucleotide and amino acid homology between 38 strains of A (H1N1) pdm09 influenza virus from 2018 to 2023 were 96.06%-100% and 96.12%-100%, respectively. From February to May 2023, all 12 strains of A (H1N1) pdm09 had two amino acid mutation sites occurring in the HA antigenic determinant cluster, namely the Ca region (A203T) and the Cb region (K71Q). No mutations were found in the HA receptor binding site and NA resistance site. The strains from January to June 2018 belong to the 6B. 1A evolutionary branch, the strains from December 2018 to January 2020 belong to three evolutionary branches: 6B. 1A. 1, 6B. 1A. 5a, and 6B. 1A. 7, and the strains from February to May 2023 belong to the 6B. 1A. 5a. 2a evolutionary branch. 38 strains of A (H1N1) pdm09 HA gene all have 7 potential N-glycosylation sites, while NA gene has 7-8 potential N-glycosylation sites.Conclusions:There are characteristic amino acid mutation sites of H1N1 influenza A in Wuxi City from 2018 to 2023. The emergence of these mutation sites may affect the virus′s transmission and antigenic changes.
