Genetic characteristics of Hantavirus detected in patients with hemorrhagic fever with renal syndrome from 2015 to 2023 in Shenzhen city
10.3760/cma.j.cn112866-20250213-00030
- VernacularTitle:深圳市2015—2023年肾综合征出血热患者汉坦病毒基因特征
- Author:
Lina WU
1
;
Yue LI
;
Yalan HUANG
;
Chunli WU
;
Dana HUANG
;
Fan YANG
Author Information
1. 南方医科大学公共卫生学院，广州 510515
- Publication Type:Journal Article
- Keywords:
Hemorrhagic fever with renal syndrome;
Hantavirus;
Genotype;
Sequence analysis;
Phylogenetic tree
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(3):324-332
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the genotype and molecular characteristics of Hantavirus causing hemorrhagic fever with renal syndrome (HFRS) in Shenzhen so as to provide basis for the prevention and control.Methods:The serum samples of HFRS patients at acute stage were collected from hospitals, and viral RNA from the sera was extracted as a template for typing detection by real-time fluorescent PCR. The nucleotide sequences of M fragment (G2 segment) and S fragment were amplified by reverse transcription-nested polymerase chain reaction (RT-nested PCR). The PCR products were then sequenced and homology and phylogenetic tree analysis were conducted.Results:In a total of 123 HV IgM antibody positive serum samples were collected in Shenzhen from 2015 to 2023, including 97 males and 26 females. Most of patients were young and middle-aged men. Cases were found throughout the year, with high incidence in spring and early summer. The result of fluorescence PCR showed that among 92 clinical specimens, 63 cases (68.5%) were tested positive for hantavirus genotypes, 59 cases (93.7% of positives) were identified as SEOV and 4 cases (6.3% of positives) as HTNV. Notably, all HTNV-positive patients were male. Analysis on nucleotide homology and phylogenetic tree showed that the difference in nucleotide sequences among SEO viruses in Shenzhen was small, and most of them were S2 subtype except for two cases of S3 subtype. The mutation rate of HTN viruses was high, with two cases of H5 subtype and two cases of H8 subtype.Conclusions:The major genotype of hantavirus causing HFRS in Shenzhen is SEO genotype, especially S2 subtype, which is closely related to some strains in Guangzhou, and has high homology with L99, Z37 and other vaccine strains. The existence of S3 subtype cases was detected for the first time in Shenzhen, while some cases of HTNV were still imported.
