Monitoring and analysis of the presence of Dabie Bandavirus in rodents in Xilingol League, Inner Mongolia Autonomous Region, China
10.3760/cma.j.cn112866-20250219-00038
- VernacularTitle:内蒙古自治区锡林郭勒盟啮齿动物携带大别班达病毒监测分析
- Author:
Xiaowei NAN
1
;
Desu WEN
;
Shanshan WANG
;
Mengmeng ZHANG
;
Xiaoyan SI
;
Zhen YAN
;
Man LUO
;
Haiyang ZHANG
;
Zhongbing ZHANG
Author Information
1. 内蒙古自治区疾病预防控制中心病媒生物防制科 内蒙古自治区预防医学科学院，呼和浩特 010031
- Publication Type:Journal Article
- Keywords:
Inner Mongolia Autonomous Region;
Pathogen transmitted by rats;
Dabie bandavirus;
Phylogenetic evolution;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(2):149-153
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the prevalence of the Dabie bandavirus among rats in Taipusi Banner, Xilingol League, Inner Mongolia Autonomous Region.Methods:The cytochrome b gene for the identification of rat species was amplified. Real-Time RT-PCR and RT-PCR were used to amplify and sequence DBV positive rat tissues and detected by XilinGol League Center for Disease Control and Prevention, and the gene evolution was analyzed.Results:The rat was identified as Spermophilus dauricus. Through molecular detection on 20 samples, 15 samples showed positive result in Real Time RT-PCR nucleic acid test, and 7 samples showed positive result in RT-PCR nucleic acid test. Seven base sequences were obtained through sequencing, and they were compared with those in the National Center for Biotechnology Information (NCBI) of the United States by BLAST. It was determined that they were the S gene sequences of DBV. It has been uploaded to GenBank with serial numbers PV231886, PV231887 and PV231888.Conclusions:DBV was detected for the first time in the mice of Inner Mongolia Autonomous Region. In future work, monitoring of DBV carried by rodents and ectoparasites such as ticks and fleas in this area should be strengthened. Publicity and education on severe fever with thrombocytopenia syndrome should be carried out in this area to improve the local medical system′s ability to identify such cases, ensuring early detection, early diagnosis, and early treatment, and reducing the occurrence of human cases and clustered outbreaks.
