Establishment of a rapid detection method for SARS-CoV-2 Omicron variant nucleic acid based on BMD-PCR technology
10.3760/cma.j.cn112866-20241212-00176
- VernacularTitle:BMD-PCR奥密克戎变异株核酸快速检测方法的建立
- Author:
Yang JIAO
1
;
Taoli HAN
;
Jianhong ZHAO
;
Shiyao ZHANG
;
Zhi LYU
;
Shaocheng WANG
;
Lingli SUN
Author Information
1. 北京市朝阳区疾病预防控制中心，北京 100021
- Publication Type:Journal Article
- Keywords:
SARS-CoV-2;
Omicron variant;
rapid detection;
BMD-PCR
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(1):109-114
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The SARS-CoV-2 has a high natural mutation rate, and dynamic monitoring of virus variants remains a key focus in current COVID-19 prevention and control efforts.Methods:In this study, a sensitive and rapid method for detecting SARS-CoV-2 omicron variant nucleic acid was established based on the BMD-PCR technology.Results:This method showed good specificity, and had no cross-reactivity with 11 common viruses transmitted via the respiratory and gastrointestinal tracts, and the limit of detection is 555 copies/ml. Compared with SARS-CoV-2 whole-genome sequencing result, among 50 samples with original Ct values ≤32 tested for the Omicron variant, 49 samples tested positive for the N679K mutation site using BMD-PCR Omicron variant detection, achieving a concordance rate of 98.00%. For 30 samples JN.1 lineage, 29 samples tested positive for the K356T mutation site using BMD-PCR JN.1 lineage detection, with a concordance rate of 96.67%. For 10 samples with original SARS-CoV-2 detection Ct values between 35 and 32, 7 samples tested positive for the N679K mutation site using BMD-PCR Omicron variant detection, Resultsing in a detection rate of 70.00%. For samples with SARS-CoV-2 nucleic acid detection Ct values>35, the detection rate for the N679K mutation site in the BMD-PCR Omicron variant was 20.00%.Conclusions:This method can serve as a high-throughput supplementary approach for the preliminary identification of SARS-CoV-2 variant genotypes.
