Mechanism of Compound Baimai Powder protecting neurons against glucose and oxygen deprivation/reoxygenation injury
10.3969/j.issn.1009-0126.2025.09.028
- VernacularTitle:复方白脉散在缺血性脑卒中保护神经元免受糖氧剥夺/复氧损伤的相关机制
- Author:
Shuyao WANG
1
;
Jia LIU
;
Yue YUAN
;
Yang LI
;
Shuhai HAN
;
Yuchuan MENG
;
Shuxia WANG
;
Jianhua BAO
;
Mo YAO
Author Information
1. 028000 通辽市人民医院神经内科
- Publication Type:Journal Article
- Keywords:
mongolian drugs;
Janus kinases;
oxygen-glucose deprivation/glucose reoxygenation
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2025;27(9):1263-1268
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect and related mechanism of Compound Baimai Powder(CBMP,a compound description of Mongolian medicine)on astrocytes after oxygen glu-cose deprivation and reoxygenation(OGD/R)injury.Methods Astrocyte model of OGD/R injury was subjected to simulate in vitro cerebral ischemia/reperfusion injury.Cultured astrocytes were randomly divided into normal group,OGD/R group,OGD/R+nimodipine group(10 μmol/L),OGD/R+low-and high-dose CBMP groups(25,50 μmol/L).Cell viability and apoptosis were de-tected with CCK-8 assay and flow cytometry,respectively.Western blotting was used to measure the expression levels of the proteins related to the nuclear factor erythroid-2-related factor-2(NRF2)/antioxidant response element and Janus kinase(J AK)/signal transducer and activator of transcription(STAT)signaling pathways.ELISA was employed to examine the levels of inflam-matory factors IL-1β,IL-6,and TNF-α,as well as oxidative stress molecules ROS,GSH,MDA and SOD.Results Compared to the normal group,the OGD/R group showed significant decreases in cell viability,NRF2 protein level,and SOD and GSH activities(P＜0.05,P＜0.01),and obvious increases in p-JAK and p-STAT proteins levels,contents of IL-1β,IL-6 and TNF-α,and ROS and MDA levels(P＜0.05,P＜0.01).High-dose CBMP treatment resulted in notably elevated cell via-bility and NRF2 protein level,while reduced levels of p-JAK[(1.20±0.20)vs(2.50±0.26)]and p-STAT[(1.15±0.25)vs(2.10±0.21)]proteins,IL-6[(30.33±5.20)vs(180.35±18.50)]and TNF-α[(50.12±8.24)vs(160.45±15.20)]when compared to the OGD/R group(P＜0.05,P＜0.01).Conclusion CBMP exerts protective effect on astrocytes against OGD/R injury.
