Effects of aloperine on the pulmonary expressions of TREM-1 and TREM-2 in septic rats
10.3969/j.issn.1001-1528.2024.10.012
- VernacularTitle:苦豆碱对脓毒血症大鼠肺组织TREM-1、TREM-2表达的影响
- Author:
Xin-yi WANG
1
;
Jing-ping XU
;
Hong KE
;
Chao XI
;
Shang-cheng XU
;
Hong DU
;
Yan-ru CUI
;
Fei QU
Author Information
1. 江西中医药大学中医学院,江西 南昌330004
- Publication Type:Journal Article
- Keywords:
aloperine;
sepsis;
acute lung injury;
TREM-1;
TREM-2;
PI3K signaling pathway
- From:
Chinese Traditional Patent Medicine
2024;46(10):3263-3270
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effects of aloperine on the pulmonary expressions of TREM-1 and TREM-2 in septic rats.METHODS The rats were randomly divided into the control group,the model group and the low,medium and high dose aloperine groups ( 25,50 and 100 mg/kg ).The corresponding intraperitoneal aloperine injection was administered 0.5 h before modeling,and the rats'Penh,Cdyn and EF50 were detected 24 hrs after modeling.The rats had their pathological injury of the lung tissue observed by HE staining;their pulmonary mRNA expressions of TREM-1,TREM-2,TNF-α,IL-1β and IL-10 detected by RT-qPCR method;and their pulmonary protein expressions of TREM-1,TREM-2,TNF-α,IL-1β,IL-10,p-p65,p-PI3K and p-Akt detected by Western blot.RESULTS Compared with the control group,the model group displayed increased Penh (P<0.01),decreased Cdyn and EF50 ( P<0.01);destroyed lung tissue structure and thickened alveolar septum,increased mRNA and protein expressions of TREM-1,TNF-αand IL-1β( P<0.05,P<0.01);decreased mRNA and protein expressions of TREM-2 and IL-10 ( P<0.05,P<0.01);and increased protein expressions of p-p65,p-PI3K and p-Akt (P<0.01).Compared with the model group,the aloperine groups shared decreased Penh ( P<0.01);increased Cdyn and EF50 ( P<0.01);improved pulmonary structure and thinner alveolar wall,decreased mRNA and protein expressions of TREM-1,TNF-α and IL-1β( P<0.05,P<0.01 );increased mRNA and protein expressions of TREM-2 and IL-10 ( P<0.05,P<0.01);and decreased protein expressions of p-p65,p-PI3K and p-Akt (P<0.05,P<0.01).CONCLUSION Aloperine can alleviate the inflammatory response of septic rats by down-regulating TREM-1 expression and up-regulating TREM-2 expression,which may be related to the inhibited activation of PI3K signaling pathway.
