Establishment of HPLC-CAD characteristic chromatograms and content determination of six carbohydrates for Qingzhi Huayu Formula

VernacularTitle:清脂化瘀方HPLC-CAD特征图谱建立及6种糖类含量测定
Author: Hai-bo ZHANG ¹ ; Qing-shuai FU ; Geng-zhen YAO ; Hua ZHOU ; Rong-sheng LI ; Tao YANG
Author Information

1. 上海中医药大学附属曙光医院心血管研究室,上海 201203;上海市中医临床重点实验室,上海201203
Publication Type:Journal Article
Keywords: Qingzhi Huayu Formula; HPLC-CAD characteristic chromatograms; carbohydrate components; quality control
From: Chinese Traditional Patent Medicine 2024;46(10):3212-3217
CountryChina
Language:Chinese
Abstract: AIM To establish the HPLC-CAD characteristic chromatograms of Qingzhi Huayu Formula,and to determine the contents of rhamnose,fructose,mannitol,D-anhydrous glucose,sucrose and raffinose.METHODS The analysis was performed on a 35 ℃ thermostatic Dikma Polyamino HILIC column (4.6 mm×250 mm,5 μm),with the mobile phase comprising of acetonitrile-water flowing at 0.8 mL/min in a gradient elution manner.RESULTS There were 14 common peaks in the characteristic chromatograms of 15 batches of samples with the similarities of more than 0.90.Six carbohydrates showed good linear relationships within their own ranges ( r>0.9950 ),whose average recoveries were 93.34％-105.64％ with the RSDs of 0.5％-2.6％.CONCLUSION This accurate,reliable,sensitive and reproducible method can be used for the quality control of carbohydrates in Qingzhi Huayu Formula.