Secondary metabolites from endophytic fungi of Taxus wallichiana var.chinensis and their biological activities
10.3969/j.issn.1001-1528.2024.10.023
- VernacularTitle:中国红豆杉内生真菌次生代谢产物及其生物活性研究
- Author:
Lei-lei CHEN
1
;
Zhong-duo YANG
1
;
Han-nan WEI
1
;
Ke-zhen MA
1
;
Wen-lin GUO
1
;
Tian ZHANG
1
Author Information
1. 兰州理工大学生命科学与工程学院,甘肃 兰州730050
- Publication Type:Journal Article
- Keywords:
Taxus wallichiana var.chinensis ( Pilger ) Florin;
endophytic fungi;
Gongronella butleri;
secondary metabolites;
isolation and identification;
PI3K
- From:
Chinese Traditional Patent Medicine
2024;46(10):3324-3329
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the secondary metabolites from endophytic fungi of Taxus wallichiana var.chinensis (Pilger) Florin and their biological activities.METHODS Endophytes were isolated and purified by tissue block and scribing method,the obtained strains were identified by molecular method.Macroporous resin column and silica gel column were used for separation and purification,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The inhibition of phosphatidylinositol 3-kinase,phosphodiesterase and fusarium oxysporum activities were determined by chemiluminescence kinase assay kit method,resazurin cell viability assay and enzyme linked immunosorbent assay.RESULTS Eight endophytic fungi were isolated and identified as Trichoderma,Aspergillus,Gongronella,Phoma,Penicillium.Strain ZGG-5 had strong PI3K inhibitory activity with IC50 value of (0.17±0.05)μg/mL,and was identified as Gongronella butleri.Thirteen compounds were isolated from ZGG-5,and compound 13 had strong activities of inhibiting PI3K at concentration of 1 mg/mL,and the inhibitory rate was (97.8±0.51)％.CONCLUSION T.wallichiana var.chinensis contains endophytic fungi with strong activities of inhibiting PI3K.Compounds 1-12 are first isolated from G.butleri.
