Simutaneous content determination of eleven constituents in different compatibility ratios of Fructus Aurantii Immaturus-Magnoliae Officinalis Cortex drug pair by QAMS
10.3969/j.issn.1001-1528.2024.12.003
- VernacularTitle:一测多评法同时测定不同配伍比例枳实-厚朴药对中11种成分的含量
- Author:
Ting HUANG
1
;
Xian-fu LIU
;
Ru-xu CHEN
;
Xia CHEN
;
Ming-yang LI
;
Cong DING
Author Information
1. 广西中医药大学,广西 南宁530222
- Publication Type:Journal Article
- Keywords:
Fructus Aurantii Immaturus;
Magnoliae Officinalis Cortex;
drug pair;
compatibility ratios;
chemical constituents;
content determination;
quantitative analysis of multi-components by single-marker (QAMS)
- From:
Chinese Traditional Patent Medicine
2024;46(12):3911-3917
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a quantitative analysis of multi-components by single-marker (QAMS) method for the simultaneous content determination of synephrine,syringin,magnoflorine,narirutin,naringoside,hesperridin,neoesperridin,limonin,naringenin,honokiol and magnolol in different compatibility ratios of Fructus Aurantii Immaturus-Magnoliae Officinalis Cortex drug pair.METHODS The ratios of 1:1,1:2 and 2:1 were adopted in the compatibility of Fructus Aurantii Immaturus and Magnoliae Officinalis Cortex,respectively.The analysis was performed on a 30℃ thermostatic Agilent 5TC-C18 column (4.6 mm × 250 mm,5μm),with the mobile phase comprising of methanol-0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.Hesperidin was used as an internal standard to calculate the relative correction factors of the other ten constituents,after which the content determination was made.RESULTS Eleven constituents showed good linear relationships within their own ranges (r≥0.9990),whose average recoveries were 97.04％-99.45％ with the RSDs of 1.22％-2.48％.The result obtained by QAMS approximated those obtained by external standard method.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Fructus Aurantii Immaturus-Magnoliae Officinalis Cortex drug pair.The extraction of various constituents is more favourable when the compatibility ratio of two medicinal materials is 1∶1.
