Optimization of stir-frying with sand process for Aconitum brachypodum and its acute toxicity,anti-arrhythmic activity
10.3969/j.issn.1001-1528.2024.12.007
- VernacularTitle:短柄乌头砂炒工艺优化及其急性毒性、抗心律失常活性研究
- Author:
Cong-gai LIU
1
;
Qing-yang ZHENG
1
;
Jia-jia CHEN
1
;
Yu-jie WANG
1
Author Information
1. 成都中医药大学,四川 成都611137
- Publication Type:Journal Article
- Keywords:
Aconitum brachypodum Diels;
stir-frying with sand process;
acute toxicity;
anti-arrhythmic activity;
Box-Behnken response surface method
- From:
Chinese Traditional Patent Medicine
2024;46(12):3938-3945
- CountryChina
- Language:Chinese
-
Abstract:
AIM To optimize the stir-frying with sand process for Aconitum brachypodum Diels.,and to investigate its acute toxicity,anti-arrhythmic activity.METHODS With frying temperature,frying time and drug-sand ratio as influencing factors,comprehensive score for total contents of diester alkaloids (aconitine,yunaconitine) and monoester alkaloids (benzoylaconitine,16-epi-pyroaconitine,8-deacetylyunaconitine,pyroyunaconitine) as evaluation indices,the stir-frying with sand process was optimized by Box-Behnken response surface method.Acute toxicity test was adopted in the calculation of LD50 of raw product and stir-frying with sand-processed product.The model for rat arrhythmia was established,after which ventricular premature beat latency and incidence of ventricular tachycardia were determined.RESULTS The optimal conditions were determined to be 210℃ for frying temperature,15 min for frying time was,and 1∶15 for drug-sand ratio,the comprehensive score was 93.932.The LD50 of raw product,stir-frying with sand-processed product were 0.076,0.799 g/kg,respectively.Compared with the model group,different doses (5,8,12,16 mg/kg) of stir-frying with sand-processed product groups demonstrated prolonged ventricular premature beat latencies (P<0.05).Compared with the 5 mg/kg dose group,the 12-16 mg/kg dose group displayed decreased incidence of ventricular tachycardia (P<0.05).CONCLUSION Reduced acute toxicity of A.brachypodum is observable after stir-frying with sand,which exhibits strong anti-arrhythmic activity at high dose.
