Drosophila microRNA dps-2526-3p suppresses dengue virus replication in Aedes aegypti cell
10.3760/cma.j.cn112866-20240816-00121
- VernacularTitle:果蝇微RNA dps-2526-3p干扰埃及伊蚊细胞中登革病毒复制的研究
- Author:
Ting CAO
1
;
Qian NI
1
;
Mengyi SHI
1
;
Xin DENG
1
;
Xiaoling PAN
1
Author Information
1. 湖南师范大学医学部医学技术与转化学院，长沙 410013
- Publication Type:Journal Article
- Keywords:
miRNA;
Dengue virus;
Aedes aegypti;
Drosophila pseudoobscura
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(6):628-634
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The toll pathway plays an essential role in the inhibition of dengue virus (DENV) replication in Aedes aegypti. Accordingly, the objective of this study was to examine the impact of microRNA on the Toll pathway regulatory genes and its influence on DENV replication in Ae. aegypti. Methods:RNAhybrid software prediction and dual-luciferase reporter gene assay were employed to study the binding relationship between dps-miR-2526-3p and Rel1 A gene. The real-time fluorescence quantitative PCR (qPCR) was used in the quantification of nuclear and cytoplasmic dps-miR-2526-3p in Wolbachia-carrying (W + ) and Wolbachia-free (W -) Ae. aegypti cells via nucleocytoplasmic separation. The miRNA up-regulation assay or miRNA silencing assay combined with virus infection assay were conducted to ascertain the impact of dps-miR-2526-3p on Rel1 A gene expression and DENV replication. Results:The result of the prediction and dual-luciferase reporter gene assay consistently demonstrated the direct binding relationship between dps-miR-2526-3p and the Rel1 A gene. The qPCR result proved the presence of dps-miR-2526-3p in the nucleus and cytoplasm of Ae. aegypti cell. Notably, the expression levels of dps-miR-2526-3p and Rel1 A gene in W + cells were significantly higher than those in W -cells. The miRNA function assay indicated that dps-miR-2526-3p could positively regulate Rel1 A gene expression. The result of viral infection assay showed that dps-miR-2526-3p exhibited a notable inhibitory effect on DENV replication. Conclusions:Ae. aegypti utilizes dps-miR-2526-3p to activate the Toll pathway via Rel1 A gene, thereby inhibiting the replication of DENV. The novel molecule with anti-DENV properties was found in this study to offer a promising approach for the development of vector control strategies aimed at prevention and control of DENV transmission.
