Isolation and characterization of an epidemic ON1 genotype of respiratory syncytial virus subtype A in Beijing
10.3760/cma.j.cn112309-20240321-00105
- VernacularTitle:北京地区1株呼吸道合胞病毒A亚型ON1基因型流行株的分离及其生物学特性研究
- Author:
Xuchang SHAN
1
;
Jiao REN
;
Wei ZHANG
;
Zhongxian ZHANG
;
Lingfang ZHANG
;
Jia LI
;
Qiaohong CHU
;
Jingdong SONG
;
Zhihai CHEN
;
Yao DENG
;
Desheng ZHAI
;
Wenjie TAN
Author Information
1. 新乡医学院公共卫生学院，新乡 453003
- Publication Type:Journal Article
- Keywords:
Respiratory syncytial virus;
Virus isolation;
Identification;
ON1 genotype
- From:
Chinese Journal of Microbiology and Immunology
2025;45(1):10-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain a prevalent respiratory syncytial virus (RSV) clinical isolate in Beijing and analyze the genotype and biological characteristics of the strain.Methods:A nasopharyngeal secretion specimen was collected from a child with RSV infection in Beijing in 2023 and used for viral isolation. Viral nucleic acid was amplified using qRT-PCR. The isolated virus was identified by transmission electron microscopy, indirect immunofluorescence assay, and plaque formation assay. A phylogenetic analysis was conducted based on the whole-genome sequencing results. Virus titers were determined, and replication characteristics were analyzed. The efficacy of the isolated strain for in vitro screening of antiviral drugs was validated. Results:A clinical RSV isolate, named hRSV/C-Tan/BJ 202301, was successfully isolated, which could form syncytia in Hep-2 cells. Spherical, filamentous, and irregular virus particles were observed by electron microscopy. Immunofluorescence detection showed green fluorescence in Hep-2 cells, and plaque assay showed round plaques, which were similar to the Long strain in morphology. Genomic sequence analysis showed that it belonged to ON1 genotype. It exhibited similar cell growth kinetics characteristics with the Long strain and could be used for antiviral drug screening in vitro. Conclusions:In this study, one RSV strain is successfully isolated and identified. The biological characteristics and the phylogenetic relationship of this strain reflect the characteristics of the circulating strains in Beijing, which provides experimental material for RSV vaccine development and antiviral drug screening in China.
